母爱剥夺对成年大鼠应激反应及艾司西酞普兰作用的影响  

Effects of maternal deprivation on stress response and efficacy of escitalopram in adult rats

作　　者：王玉婷[1,2] 王雷[1] 张逸[1] 王鑫[1] 朱熊兆[1] Wang Yuting Wang Lei Zhang Yi Wang Xin Zhu Xiongzhao(Medical Psychology Center, the Second Xiangya Hospital, Central South University, Changsha 410011, China Department of Clinical Psychology,Hunan Provincial Maternal and Child Health Care Hospital, Changsha 410008, China)

机构地区：[1]中南大学湘雅二医院医学心理学研究所,长沙410011 [2]湖南省妇幼保健院临床心理研究室,长沙410008

出　　处：《中华行为医学与脑科学杂志》2017年第3期198-203,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81071097）

摘　　要：目的研究经历过早年母爱剥夺的大鼠在其成年期遭遇慢性应激时的行为反应及其特征；探讨早年母爱剥夺对艾司西酞普兰抗抑郁作用的影响。方法以雄性SD大鼠为对象，采用随机数字表法分为4组：空白对照（C）组、母爱剥夺（MD）组、慢性不可预知性应激（CUPS）组、母爱剥夺合并慢性不可预知性应激（MD＋CUPS）组；MD组和MD＋CUPS组出生第1天开始接受14d的母爱剥夺，CUPS组和MD＋CUPS组于第10周开始接受28d的CUPS处理；第14周开始对不同应激致抑郁的大鼠进行4周的艾司西酞普兰抗抑郁治疗。结果4组大鼠的快感缺失发生率差异有统计学意义（Х^2=143．24，P〈0．01），依次为：MD＋CUPS组（75．76％）〉CUPS（40．98％）组〉MD（17．11％）组〉C（4．17％）组，组间两两比较差异有统计学意义（均P〈0．0083）；4组大鼠的行为绝望发生率差异有统计学意义（Х^2=70．34，P〈0．01），MD＋CUPS（43．43％）与CUPS（39．34％）组大鼠的行为绝望发生率差异无统计学意义，但二者均高于MD组（13．51％）及C组（3．33％），MD组显著高于C组（P〈0．0083）；艾司西酞普兰对3种不同应激致大鼠快感缺失和行为绝望的治愈率组间差异均无统计学意义，但艾司西酞普兰对快感缺失的总治愈率（44／140）显著低于行为绝望的总治愈率（76／140）（Х^2=14．93，P〈0．05）。结论母爱剥夺增加了成年大鼠的应激敏感性及快感缺失的发生率；母爱剥夺不影响艾司西酞普兰的抗抑郁作用，但艾司西酞普兰对不同抑郁行为的作用存在差异。Objective To investigate the effects of maternal deprivation on chronic stress-induced depression behavior and its characteristics in adult rats, and to evaluate the effects of maternal deprivation on the efficacy of escitalopram. Methods Newborn SD male rats were randomly divided into control （C） group, maternal deprivation （MD） group, chronic unpredictable stress （CUPS） group, maternal deprivation and chronic unpredictable stress （MD＋CUPS） group. Rats in control group received no experimental handling.Rats in MD group and MD＋CUPS group received maternal deprivation from the 1st day after birth for 14 days.Rats in CUPS group and MD＋CUPS group received chronic unpredictable stress from 10 th weeks after birth for 28 days. Screened the rats with depression behaviors and treated them with escitalopram for 4 weeks. Results The incidence of anhedonia was significantly different among 4 groups （Х^2 = 143.24, P〈 0.05 ）. Pairwise comparison showed significant difference between any two groups （MD＋CUPS group （75.76%） 〉 CUPS group （40.98%） 〉 MD group （17.11%） 〉C group （4.17%） , P〈0.0083）. The incidence of behavioral despair was significantly different among 4 groups （Х^2= 70.34, P〈0.05 ）. Pairwise comparison showed the incidence of behavioral despair in MD＋CUPS group （43.43%） and CUPS group （39.34%） were significantly higher than that in MD group （13.51%） and C group （3.33%）,but no difference was observed between MD＋CUPS and CUPS group （P〈0.0083）. The incidence of behavioral despair in MD group was significant higher than that in C group. There was no significant efficacy of escitalopram on anhedonia and behavioral despair among 3 stressed models. However the recovery incidence from anhedonia （44/140） was significantly lower than that from behavioral despair （76/140） （Х^2= 14.93, P〈0.05）. Conclusion The maternal deprivation increases the stress sensitivity and the incidences of anhedonia in adult rats.The efficacy of

关 键 词：母爱剥夺 慢性不可预知性应激 快感缺失 行为绝望 艾司西酞普兰 

分 类 号：R749.4[医药卫生—神经病学与精神病学]