2种低温保藏法对金黄壳囊孢菌种活力及致病力的影响  

Effect of 2 Methods with Low-temperature Preservation on Strains Viability and Pathogenicity of Cytospora chrysosperma

作　　者：张静文[1] 杨寒丽[2] 孔婷婷[1] 刘爱华[1] 岳朝阳[1] 焦淑萍[1] 

机构地区：[1]新疆林业科学院森林生态研究所,新疆乌鲁木齐830000 [2]新疆巴州农业技术推广中心,新疆库尔勒841000

出　　处：《西南林业大学学报（自然科学）》2017年第2期158-162,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：新疆维吾尔自治区公益性科研院所基本科研业务经费项目(XMBM000003014)资助

摘　　要：为研究不同方法长期保存金黄壳囊孢菌种的活力和致病力,分别对1986年石蜡油低温保存和2009年试管斜面低温保存菌株进行检测。结果表明:石蜡油低温保存菌株的平均活力指数为33.3%,试管斜面低温保存菌株的平均活力指数为10.0%;石蜡油低温保存金黄壳囊孢菌的存活期长达29 a,试管斜面低温保存也有较高的成活率;2种方法保存的金黄壳囊孢菌株都存在致病活性,但是致病力有一定的差异,石蜡油低温保存菌株的致病力高于试管斜面低温保存的菌株;林间接种石蜡油低温保存菌株,新疆杨发病率最高可达40%。In this study, lowtemperature preserved of Cytospora chrysosperma in paraffin oil from 1986s and preserved in test-tube bevel from 2009s, were tested for their viability and pathogenicity. The results showed that the average viability index of C.chrysosperma preserved by paraffin oil was 33.3%, the average viability index of the strain preserved by test-tube bevel was 100%. Therefore, the longest preservation time was 29 years by paraffin oil in lowtemperature. The test-tube bevel preservation also has a higher survival rate. There were some differences in the pathogenicity of the 2 methods for the preservation of C.chrysosperma. The pathogenicity of the strains of low temperature preservation by paraffin oil were higher than that of test-tube bevel. In the field, the incidence disease of Populus bolleana was up to 40%, while inoculated with strains of low temperature preservation by paraffin oil.

关 键 词：金黄壳囊孢 活力指数 致病力 菌种保存 

分 类 号：S763.15[农业科学—森林保护学]