补肾益髓方及其拆方对实验性自身免疫性脑脊髓炎小鼠脑和脊髓中p-Tau和Beta-tubulin表达的影响  

Effects of Bushen Yisui Decoction and Its Decomposed Decoctions on Expressions of p-Tau and Beta-tubulin in Brain and Spinal Cord of Mice with Experimental Autoimmune Encephalomyelitis

作　　者：师一民 王永强[1] 安辰[1] 赵晖[1] 齐放[1] 张秋霞[1] 陈振振[1] 樊永平[2] 李明[1] 王蕾[1] 

机构地区：[1]首都医科大学中医药学院中医络病研究北京市重点实验室,北京100069 [2]首都医科大学附属北京天坛医院,北京100050

出　　处：《世界中医药》2016年第9期1825-1830,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81273742;81573898);北京市教委重点项目(编号:KZ201310025023);北京市属高等学校高层次人才引进与培养计划-长城学者项目(编号:CIT&TCD20140329)

摘　　要：目的:观察补肾益髓(Bu Shen Yi Sui,BSYS)方及其拆方补肾(Bu Shen,BS)和化痰活血(Hua Tan Huo Xue,HTHX)方对实验性自身免疫性脑脊髓炎(Experimental Autoimmune Encephalomyelitis,EAE)小鼠脑和脊髓中p-Tau和Beta-tubulin的作用。方法:C57BL/6小鼠雌性随机分为正常对照(NC)、模型(MO)、醋酸泼尼松(PA)、梓醇(CA)、BSYS、BS和HTHX组。造模当天与造模后第7天于小鼠背部两侧皮下注射抗原,即50μg髓鞘少突胶质细胞糖蛋白(MOG)35-55、完全弗氏佐剂(CFA)与结核分支杆菌(TB)混合,并在免疫当天和2 d后腹腔注射500 ng的百日咳毒素(PTX)。每天对小鼠进行灌胃,观察其体重及神经功能评分,并于造模第20 d和第40 d分别取脑和脊髓进行病理检测。采用免疫组化法检测p-Tau和beta-tubulin的表达。结果:与MO组比较,BSYS、BS和HTHX组体重与神经功能评分均出现明显上升,HE染色可观察到炎细胞显著减少,核固缩减轻,同时,BSYS、BS和HTHX方均可明显下调p-Tau表达(P<0.05,P<0.01),上调beta-tubulin蛋白表达(P<0.05,P<0.01)。结论:BSYS方及其拆方BS和HTHX均对EAE小鼠有神经保护作用,表现在减少体重丢失,降低神经功能评分,减轻炎炎性细胞浸润,减轻轴突损伤及促进其修复,尤以BSYS全方更为显著。Objective： To observe effect of the Bushen Yisui Dection（ BYD） and its decomposed Bushen Decoctions（ BD） and Huatan Huoxue Decoction（ HHD） on the expressions of p-Tau and beta-tubulin in the brain and spinal cord of mice with experimental autoimmune encephalomyelitis（ EAE）. Methods： C57 BL /6 female mice were randomly divided into normal control（ NC）,model（ MO） and prednisone acetate（ PA）,catalpol（ CA）,BYD,BD and HHD groups. The mice were injected subcutaneously with the antigens mixture,including 50 μg OG35-55,CFA and TB at two sites on the back on the 1st day and 7 days later,and then were injected intraperitoneally with 500 ng PTX on days 1st and 2 day later. The mice in each group were administered intragastrically once a day. Meanwhile,the body weight and neurological function of mice were observed. The brain and spinal cord of mice were removed for pathological examination on 20 th and 40 th day. The expressions of p-Tau and beta-tubulin were detected by immunohistochemistry analysis. Results： Compared with the MO group,the body weight and scores of mice were significantly increased in BYD,BD and HHD groups. HE staining showed a significant reduction in inflammatory cells and nuclear condensation. The expression of p-Tau was significantly reduced,while beta-tubulin was markedly increased in BYD,BD and HHD groups as well（ P〈0. 05,P〈0. 01）. Conclusion： BYD and its decomposed decoctions BD and HHD have neuroprotective effects on reducing weight loss and scores,inflammatory cell infiltration,axonal injury and enhancing the repair of axon in EAE mice. The effect of Bushen Yisui Decoction is more significant particularly.

关 键 词：补肾益髓方及其拆方 多发性硬化 实验性自身免疫性脑脊髓炎 P-Tau BETA-TUBULIN 

分 类 号：R744.3[医药卫生—神经病学与精神病学]