免疫复合法建立大鼠溃疡性结肠炎模型的探讨  

Discussion on Establishment of Experimental Rats Model with Ulcerative Colitis by Immune Complex Method

作　　者：常孟然 林燕[1] 李文静[1] 赵程博文 王红彬[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《世界中医药》2016年第10期2086-2088,2093,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81202682)--基于线粒体能量代谢探讨溃疡性结肠炎脾虚为本的病理基础

摘　　要：目的:探讨免疫复合法和化学法建立大鼠溃疡性结肠炎模型的优劣,寻求更接近人类发病机制的溃疡性结肠炎动物模型。方法:将Wistar大鼠随机分成正常组、免疫复合组和化学组,免疫复合组前3周每周给予抗原乳化液(含异体抗原8 mg)注射1次,共注射3次,第4周免疫复合组和化学组均用100 mg TNBS/kg+50%乙醇灌肠。分别于造模后第1天、第14天、第56天取材,比较造模后各组大鼠的症状、体征、病理反应等方面的变化。结果:与正常组比较,免疫复合组与化学组在造模后第1天均出现稀溏便、溃疡、炎性反应等典型溃疡性结肠炎病变,但免疫复合组黏膜下层炎性反应剧烈,维持时间长达8周,化学组溃疡较免疫复合组深,但黏膜下层炎性反应强度不及免疫复合组,溃疡持续时间短,2周后趋向自愈。结论:免疫复合法模型表现稳定,重复性好,病变持续时间长,发病机制更类似人类溃疡性结肠炎,是较为理想的溃疡性结肠炎模型。Objective： To screen an optimal ulcerative colitis animal model more close to the pathogenesis of human body by analysis of the strength and weakness between the immune complex method and the chemical method for establishing an experimental rat model. Methods： Wistar rats were randomly divided into normal group,immune complex group and chemical group. Rats in immune complex group are injected with antigen emulsion（including 8 mg variant antigen） once a week in the first three weeks.In the fourth week,the rats in the immune complex group and those in the chemical group were coloclystered by TNBS（2,4,6-trinitrobenzenesulfonic acid sol,100 mg/kg） and 50% alcohol. Colon samples from the three groups were taken at the 1 st,14 th and 56 th day respectively and symptom,sign and pathological reaction of the three groups after modeling were compared. Results：Compared with the normal group,rats in the immune complex group and chemical group had typical ulcerative colitis symptoms at the 1 st day,like loose stool,ulcer,inflammatory reaction,while the submucosa inflammatory reaction of rats in the immune complex group was stronger than that of the chemical group lasting for eight weeks. The ulcer in the chemical group was more severe than that of the immune complex group,but submucosa inflammatory reaction weaker,with less duration and self-cures two weeks later. Conclusion： The immune complex model is the optical ulcerative colitis one,for stable performance,easily repeated,and long duration and the ulcerative colitis pathogenesis in rat model is much more close to that of human body.

关 键 词：免疫复合法 化学法 溃疡性结肠炎 大鼠模型 

分 类 号：R574.62[医药卫生—消化系统] R285.5[医药卫生—内科学]