草酸钙型肾结石小鼠模型的研究  

Study of Calcium Oxalate Crystallization Model in Mice

作　　者：曾春晖[1] 李先梅[1,2] 蔡妮娜[1,3] 谭娥玉[1,4] 覃文慧[1] 韦乃球[1] 秦树森 马雯芳[1] 杨柯[1] 

机构地区：[1]广西中医药大学药学院,南宁530001 [2]广西钦州市卫生学校,钦州535000 [3]南宁市中医医院,南宁530001 [4]江门市五邑中医院,江门529000 [5]桂林市妇女儿童医院,桂林541001

出　　处：《世界中医药》2016年第11期2213-2215,共3页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81260667);广西科技厅面上项目(编号:2011GXNSFA018204);广西卫生厅中医药科技专项课题(编号:GZKZ10-050);广西重点实验室建设项目(编号:15-140-31;14-045-12;13-051-06);广西农作物废弃物功能成分研究协同创新中心建设(编号:CICAR2015-Z1)

摘　　要：目的：摸索简单易行的方法诱导草酸钙型肾结石小鼠模型,为后续药效学研究提供可靠的动物模型。方法：将健康小鼠分为正常组（A组）、模型组（B组）、模型组（C组）,B组灌胃给予诱石剂（含0.75%乙二醇和0.75%氯化铵的水溶液）,C组灌胃给予诱石剂（含1%乙二醇和1%氯化铵的水溶液）,1 m L/次,3次/d,连续28 d进行造模,A组平行灌胃给予等量生理盐水。实验期间观察小鼠的进食进水情况,生命体征,行为活动及体毛变化,于造模后7、14、21、28 d收集24 h尿液进行草酸和钙含量的测定,于末次造模后处死小鼠,剖取肾脏进行病理学观察。结果：于造模第3天开始,B、C组小鼠均出现后肢无力爬行,食欲减退,体毛呈进行性晦暗无光泽,C组小鼠的表现比B组小鼠严重;造模7 d后,模型组开始出现不同数量的动物死亡,且C组死亡率高于B组;B组和C组小鼠尿液中草酸、钙浓度均明显高于A组（P〈0.01）,B组尿草酸浓度明显高于C组（P〈0.05~0.01）,而尿钙浓度明显低于C组（P〈0.01）;病理学结果表明,A组小鼠肾小管未见扩张和草酸钙晶体沉积,B组、C组小鼠肾组织均出现肿胀,肾小管管腔明显扩张、变性、坏死,肾间质有明显慢性炎性细胞浸润,表面点状分布白色结晶或钙化,在近曲小管和远曲小管有大片结晶存在,且B组的成石率比模型C组高。结论：采用0.75%乙二醇及0.75%的氯化铵混合溶液作为诱石液,可成功诱导死亡率低、成石率高的草酸钙型肾结石小鼠模型。Objective：To explore a method of establishing mice model with calcium-oxalate kidney stones so as to provide the reli-able animal models for follow-up study on pharmacodynamics .Methods： Mice were divided to normal group （ A group ） , model group （B group） and model group （C group） randomly.0.75%ethylene glycol and 0.75% ammonia chloride mixture were ad-ministrated orally1 mL/time and 3 times/day to B group mice for 28 consecutive days .1%ethylene glycol and 1%ammonia chlo-ride mixture were administrated orally to C group mice as well as B group .The same volume of normal saline was administered in parallel to A group.Food and water intake, vital signs, behavior and change of hair were observed in experiment .24 h urine of mouse was collected for detecting concentration of oxalic acid and calcium at 7, 14, 21, 28 days after modeling.All of mice were sacrificed at the last time , and took the kidney to carry out pathological observation .Results：The phenomenon of inability to crawl on the hind legs , loss of appetite and the dull hair were observed in B and C group mice after modeling 3 days.The situation of C group was more serious than B group .The model animals began to die after modeling 7 days and the mortality rate of C group was higher than that of group B .The concentration of oxalic acid and calcium in urine of B group or C group were more than A group （P〈0.01）.The concentration of oxalic acid in B group was significantly higher than that in C group （P〈0.05~0.01）, but the calcium concentration was significantly lower than that of the C group （ P〈0.01 ） .No abnormalities and Calcium oxalate crystal were found in the kidney of A group mice .In B and C groups , the renal tissues of mice were swollen .The renal tubular lumen showed obvious expansion , degeneration necrosis and there was obvious chronic inflammatory cell infiltration in renal interstitial . The surface of the kidney is dotted with white crystals or calcification .There are large tracts of crystal in the prox

关 键 词：草酸钙型肾结石 小鼠 动物模型 

分 类 号：R36[医药卫生—病理学]