法舒地尔对SH-SY5Y细胞氧糖剥夺后突触损伤的影响  

Synaptic Protective Effect of Fasudil on SH-SY5Y Cells Synapses after Oxygen-glucose Deprivation

作　　者：张伟[1,2] 张海飞[2] 马杰[1,2] 赵静[1,2] 柴智[3] 刘建春[3] 宋丽娟[3] 姜维佳 肖保国[3,4] 马存根[1,3] 

机构地区：[1]山西医科大学第一临床医学院神经内科,太原030001 [2]大同市第五人民医院神经内科,山西037009 [3]山西中医学院“2011”协同创新中心/神经生物学研究中心,太原030024 [4]复旦大学华山医院神经病学研究所,上海200025

出　　处：《神经损伤与功能重建》2017年第2期99-102,共4页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金2012年面上项目(No.81272163);山西省回国留学人员重点科研资助项目(No.2014-重点7);山西中医学院“2011”培育计划项目(No.2011PY-1)

摘　　要：目的:探讨法舒地尔(Fasudil)对人神经母细胞瘤细胞(SH-SY5Y)氧糖剥夺(OGD)后突触损伤的影响。方法:培养SH-SY5Y细胞,分为空白对照组、OGD组、OGD+法舒地尔组,各3皿。相差光学显微镜下观察细胞突触损伤及修复的细胞形态;Western-Blot检测ROCKⅡ、磷酸化肌球蛋白磷酸酶(p-MYPT1)、突触后致密物-95(PSD-95)、突触素(Synaptophysin)等蛋白的表达情况。结果:形态学显示法舒地尔可有效修复OGD诱导的神经突触损伤。OGD组ROCKⅡ及p-MYPT1的表达明显高于空白对照组(P<0.05),突触素及PSD-95的表达明显低于空白对照组(P<0.01)。OGD+法舒地尔组ROCKⅡ及p-MYPT1表达水平低于OGD组(P<0.05),而与对照组差异无统计学意义(P>0.05);突触素和PSD-95表达水平高于OGD组(P<0.01);PSD-95表达水平高于空白对照组(P<0.05),突触素的表达与空白对照组差异无统计学意义(P>0.05),结论:法舒地尔可有效修复OGD诱导的神经突触损伤,增加PSD-95、突触素的表达,抑制ROCKⅡ及p-MYPT1的表达。Objective： To investigate the effect of Fasudil on synaptic regeneration and repair of SH-SY5Y cells after oxygen-glucose deprivation （OGD）. Methods： SH-SY5Y cells were cultured, and randomly divided into three groups： control group, OGD group and OGD＋Fasudil group. The morphology of synaptic injure and re- pair was observed by phase contrast microscopy, and the expressions of ROCKII, phosphorylated myosin phos- phatase 1 （p-MYPT1）, postsynaptic density （PSD）-95 and synaptophysin were evaluated by western blot assay. Results： Compared with those in the control group, the cytological morphology and synapses were significantly injured in the OGD group, which were obviously restored to the normal state in the OGD＋ Fasudil group. Cells in the OGD group had higher expressions of ROCKII and p-MYPT1 （P〈0.05） and lower expressions of PSD-95 and synaptophysin （P〈0.01） than those in the control group. Cells in the OGD＋Fasudil group had lower expres- sions ROCKII and p-MYPT1 （P〈0.05） and higher expressions of PSD-95 and synaptophysin than those in the OGD group （P〈0.01）. The expression of PSD-95 in the OGD＋Fasudil group was higher than that in the control group （P〈0.05）. The expressions of ROCKⅡ, p-MYPT1 and synaptophysin showed no differences between the OGD＋Fasudil group and control group （P〉0.05）. Conclusion： Fasudil promots synaptic regeneration and repair of SH-SY5Y cells after OGD condition. Fasudil increases the expressions of PSD-95 and synaptophysin, as well as decreases expressions of ROCKII and p-MYPT 1 in SH-SY5Y cells.

关 键 词：法舒地尔 SH-SY5Y细胞 氧糖剥夺 ROCKⅡ 磷酸化肌球蛋白磷酸酶 突触后致密物-95 突触素 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]