基于“单细胞移植-成瘤性验证”模式鉴定肝癌干细胞  

A preliminary establishment of the “single-cellt-ransplant-tumorigenic-test” model for identifying liver cancer stem cells

作　　者：贾微[1] 岑妍慧[1] 包鹃 杨瑞[1] 何国珍[1] 吴晓君[1] 李熠毅[1] 

机构地区：[1]广西中医药大学,广西壮族自治区南宁市530222

出　　处：《中国组织工程研究》2017年第9期1324-1328,共5页Chinese Journal of Tissue Engineering Research

基　　金：the National Natural Science Foundation of China,No.81503406;University Research Projects in Guangxi,No.ZD2014068;Project of Improving the Basic Ability of Young Teachers in Universities in Guangxi,No.KY2016YB218,KY2016YB224;Special Scientific Project of Traditional Chinese Medicine supported by Health Department of Guangxi Zhuang Autonomous Region,No.GZPT13-04,GZBZ16-07,GZLC16-23;Student Research Training Project of Guangxi Medical University,No.2014DXS02~~

摘　　要：背景:目前尚无较有效的肝癌干细胞的鉴定方法。目的:探索更为简单有效并适用于鉴定肝癌肿瘤细胞株中干细胞的方法。方法:首先利用单细胞分离技术获得单个细胞,逐个种入96孔板中,经筛选获得单细胞克隆(即成瘤性克隆)来源的细胞亚株,细胞亚株移植到裸鼠前肢腋下观察其成瘤作用。结果与结论:利用单细胞分离技术获得单细胞/孔的孔数为371个,成功率为96.4%;按照单细胞克隆的生长情况,筛选获得的来源于成瘤性克隆的细胞亚株移植到裸鼠前肢腋下,成瘤率为100%。结果证实"单细胞移植-成瘤性验证"具有鉴定肝癌干细胞的有效性,为后续的研究奠定技术和方法基础。BACKGROUND：There is no effective method to identify liver cancer stem cells until now,which has become one of the major challenges in this field.OBJECTIVE：To explore a more effective and suitable way for the identification of liver cancer stem cells in hepatocellular carcinoma cell lines. METHODS：Firstly,the single cell separation technique was used to obtain single cells,which were seeded into96-well plates one by one.Secondly,cell sublines derived from single cell colonies（tumorigenic colonies）were selected and obtained.At last,the cells from these clones were transplanted into the forelimb armpits of nude mice to observe the tumorigenic ability.RESULTS AND CONCLUSION：The number of holes of single cells obtained was 371 by the single cell separation technique,and the success rate was 96.4%.According to the growth of cell clone,tumorigenic colonies were selected to be transplanted to the forelimb armpits of nude mice,and the tumor formation rate of the colonies was 100%.The identification model of＂single-cell-transplant-tumorigenic-test＂for liver cancer stem cells is confirmed to be preliminarily established,which lays the technology and methodological basis for the follow-up research.

关 键 词：HEPG2细胞 肿瘤干细胞 组织工程 干细胞 单细胞移植-成瘤性 肝癌干细胞 干细胞鉴定 HEPG2肝癌细胞 裸鼠 细胞移植 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]