结直肠癌组织中CD44^+/SOX2^+肿瘤干细胞的分布及形态特征  

Distribution and morphological characteristics of CD44^+/SOX2^+ cancer stem cells in colorectal carcinoma

作　　者：袁杭[1,2] 刘斌[1,2,3,4] 铁娅滕 孙亚敏[1,4] 辛晴[1,4] 苏勤军[1] 李双明[1] 

机构地区：[1]解放军兰州军区兰州总医院病理科,甘肃省兰州市730050 [2]兰州大学基础医学院病理研究所,甘肃省兰州市730030 [3]甘肃省干细胞与基因药物重点实验室,甘肃省兰州市730050 [4]甘肃中医药大学基础医学院,甘肃省兰州市730000

出　　处：《中国组织工程研究》2017年第9期1329-1333,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:CD44是常用的结直肠癌肿瘤干细胞标志物,近年来发现转录因子SOX2是潜在的结直肠癌肿瘤干细胞标志物。目的:观察CD44^+/SOX2^+细胞在结直肠癌组织中的数量、分布及形态特征,探讨CD44^+/SOX2^+是否可作为结直肠癌肿瘤干细胞标志物。方法:运用免疫组织化学双重染色方法观察10例正常肠黏膜、20例结直肠腺瘤及88例癌组织标本中CD44^+/SOX2^+细胞的数量、分布规律;苏木精-伊红染色观察结直肠癌组织中CD44^+/SOX2^+细胞的形态特征。结果与结论:(1)正常肠黏膜中未见到CD44^+/SOX2^+细胞,结直肠腺瘤组织中可见极少量CD44^+/SOX2^+细胞,结直肠癌中可见少量CD44^+/SOX2^+细胞;(2)CD44^+/SOX2^+细胞在高、中分化腺癌中呈小灶性或点状散在分布于隐窝基底部或共壁腺管侧,在低分化腺癌中呈小灶性或点状散在分布于其他癌细胞中;(3)在苏木精-伊红染色切片中CD44^+/SOX2^+细胞体积小,呈圆形、椭圆形结构,胞浆少,核大,核仁明显,核分裂象少见;(4)CD44^+/SOX2^+的表达与结直肠癌患者年龄、性别、部位及浸润深度均无相关性,与分化程度呈负相关,与TNM分期、远处转移均呈正相关;(5)结果提示,CD44^+/SOX2^+细胞的分布、形态特征等符合肿瘤干细胞特性,CD44^+/SOX2^+可能是结直肠癌肿瘤干细胞标志物。BACKGROUND： CD44 is commonly used as a marker of colorectal cancer stem cells. In recent years, it is found that transcription factor SOX2 is a potential marker of colorectal cancer stem cells. OBJECTIVE：To observe the number,distribution and morphological characteristics of CD44＋/SOX2＋cells in colorectal cancer,and to explore whether CD44＋/SOX2＋is a marker of colorectal cancer stem cells.METHODS：Immunehistochemical double staining method was used to observe the number and distribution of CD44＋/SOX2＋cells in 10 cases of normal intestinal mucosa,20 cases of colorectal adenoma and 88 cases of cancer tissue specimens.Hematoxylin-eosin staining was used to observe the morphological characteristics of CD44＋/SOX2＋cells in colorectal cancer tissues.RESULTS AND CONCLUSION：Immunehistochemical double staining showed that：there were no CD44＋/SOX2＋cells in normal intestinal mucosa,a very small amount of CD44＋/SOX2＋cells in colorectal adenoma,and a small amount of CD44＋/SOX2＋cells in colorectal carcinoma.The CD44＋/SOX2＋cells in highly and moderately differentiated adenocarcinoma were scattered or distributed focally in the basal part of crypt or along the co-walled gland.In poorly differentiated adenocarcinoma,they were scattered or distributed focally in other cancer cells.Hematoxylin-eosin staining showed that the CD44＋/SOX2＋cells were small,round or oval structure,with less cytoplasm,large nuclear,obvious nucleolus,and the mitotic phenomenon was rarely seen.There was no correlation between the expression of CD44＋/SOX2＋and age,gender,location,depth of invasion in colorectal cancer patients;there was a negative correlation with differentiation and a positive correlation with TNM staging,distant metastasis.To conclude,CD44＋/SOX2＋cells are in line with the characteristics of tumor stem cells in terms of distribution and morphology character,and CD44＋/SOX2＋may be a marker of colorectal cancer stem cells.

关 键 词：结直肠肿瘤 肿瘤干细胞 抗原 CD44 SOX转录因子 组织工程 干细胞 结直肠癌 CD44 SOX2 组织芯片 免疫组织化学双重染色 

分 类 号：R394.2[医药卫生—医学遗传学]