AIH治疗失败后行IVF-ET患者卵泡液中抗精子抗体和抗心磷脂抗体表达情况及妊娠结局  

Relationship between pregnancy outcome and expression of AsAb and AcAb in follicular fluid in IVF-ET patient after AIH failure

作　　者：徐楗荧[1] 赵雪燕[1] 王磊[1] 杨嫦玉[1] 蔡桂丰[1] 

机构地区：[1]珠海市妇幼保健院生殖中心,珠海519000

出　　处：《生殖医学杂志》2017年第4期341-344,共4页Journal of Reproductive Medicine

基　　金：珠海市卫生局科研项目(项目编号:2014018)

摘　　要：目的探讨夫精人工授精(AIH)治疗失败后行IVF-ET患者,卵泡液中抗精子抗体(AsAb)和抗心磷脂抗体(AcAb)表达情况及妊娠结局。方法回顾性分析2014年1月~10月在我院生殖中心AIH≥3次失败后行IVF-ET的79个周期,其中30个周期行IVF-ET后无优质胚胎移植或未妊娠者纳入A组,49个周期行IVF-ET后成功妊娠者作为对照B组,比较两组临床资料、获卵、受精及优质胚胎情况;应用酶联免疫吸附试验(ELISA)方法检测两组卵泡液中AsAb和AcAb的表达。结果两组年龄、不孕年限、体重指数(BMI)、激素等临床资料比较,差异均无统计学意义(P>0.05)。A组受精率和优质胚胎数显著低于B组(P<0.01);A组平均获卵数和卵裂率低于B组,但差异无统计学意义(P>0.05)。卵泡液中AsAb和AcAb在A组阳性率均显著高于B组(P<0.01)。结论AIH≥3次未妊娠后行IVF-ET卵泡液中AsAb和AcAb阳性表达,可能影响受精、优质胚胎形成和胚胎种植生殖环节。Objective： To investigate the relationship between pregnancy outcome and the expression of antisperm antibodies （AsAb）and anticardiolipin antibodies （AcAb） in follicular fluid in the IVF-ET patients after the failure of assisted insemination with husband sperm （AIH）. Methods： The data of 79 IVF-ET cycles of infertile patients after ≥3 times of AIH failure in the reproductive medical centre of our hospital from Jan. to Oct. 2014 were analyzed. Thirty cycles had no high-quality embryos transfer or no pregnancy in group A,and 49 cycles had successful pregnancy in group B. The clinical data, number of oocytes retrieved, fertilization rate and number of high-quality embryos were compared between the two groups. The expressions of AsAb and AcAb protein in follicular fluid were determined by enzyme linked immunosorbent assay （ELISA） and compared between the two groups. Results： There was no statistically differences in age, duration of infertility, body mass index （BMI） and hormone levels （P〉0.05）. The fertilization rate and high-quality embryo number in group A were significantly lower than those in group B （P〈0.0l）. The number of oocytes retrieved and cleavage rate were lower in group A than those in group 13, but the differences were not significant （P〉0.05）. The positive rates of AsAb and AcAb in group A were significantly higher than those in group 13 （P〈0.01）. Conclusions： The positive expression of AsAb and AcAb in follicular fluid had negative effects on the fertilization, formation of high-quality embryo and embryo implantation in IVF-ET patients after ≥ 3 times of AIH failure.

关 键 词：夫精人工授精 体外受精-胚胎移植 抗精子抗体 抗心磷脂抗体 妊娠结局 

分 类 号：R[医药卫生]