MicroRNA-223表达与肝癌根治性切除术预后的相关性  

Value of miRNA-223 expression in predicting outcome of hepatocellular carcinoma after radical therapy

作　　者：王云雀[1] 黄湘壹 

机构地区：[1]武汉理工大学校医院,湖北武汉430070 [2]南华大学附属第二医院,湖南衡阳421001

出　　处：《中国现代医学杂志》2017年第6期59-64,共6页China Journal of Modern Medicine

摘　　要：目的识别可以作为肝癌根治性切除术患者预后标志物的microRNA(miRNA)分子。方法利用miRNA微阵列方法对2例原发性肝癌及对应的肺转移性肝癌进行miRNA表达分析。将原发性和转移性肝癌中表达量有2倍差异的miRNAs作为候选miRNAs分子。在miRNA验证过程中,运用实时荧光定量聚合酶链反应(qRT-PCR)分析37例肝癌及相应癌旁非肿瘤组织中候选miRNAs分子的表达,并将其与患者的临床病理特征及生存情况进行相关性分析。结果最终选取8种miRNA(smiR-27b、122、142-5p、196a、223、590-5p、630及944)作为候选miRNA。只有miR-223表达水平与肿瘤分期、有丝分裂指数相关。将肝癌患者分为miR-223高表达组(25例)和miR-223低表达组(12例)后发现,miR-223高表达与较高T分期、淋巴结转移、较高丝分裂指数及较高Ki-67阳性指数相关。此外,miR-223高表达还与总体生存率和无病存活率下降相关(P<0.05),中位随访时间37.9个月[疾病复发危险比为16.267(95%CI:1.732,153.789)P=0.015]。结论肝癌组织中miR-223异常表达与肝癌患者治疗结束后发生复发转移,以及无病生存时间和总生存率有关,通过检测肿瘤组织中miR-223 qRT-PCR反应有可能预测患者预后。Objective To identify miRNAs that could be potential prognostic biomarkers of hepatocellular carcinoma in patients who underwent radical resection. Methods For miRNA target screening, 2 primary HCC and the corresponding liver metastases were screened for miRNA expression by the miRNA Chip analysis. Candidate miRNAs were selected by more than 2-fold difference of expression between metastatic and primary tumors. For miRNA target validation, qRT-PCR was performed for candidate miRNAs on 37 HCC and the matched paracancerous non-tumor tissues, and the correlations of the miRNA levels with the clinicopathologi-cal features and survival were analyzed. Results Eight miRNAs （miRNA-27b, 122, 142-5p, 196a, 223, 590-5p, 630, and 944） were selected as candidate miRNAs. Only miR-223 level was significantly associated with tumor stage and mitotic count. When the HCC patients were stratified into high （25 cases） and low （12 cases） miRNA-223 expression groups, high miRNA-223 expression was associated with advanced T stage, lymphatic metasta-sis, higher mitotic counts, and higher Ki-67-labeling index. In addition, high miRNA-223 expression was as-sociated with decreased overall survival and tumor-free survival （P〈0.05） during a median followup of 37.9 months [the hazard ratio for recurrence=16.267 （95%CI： 1.732, 153.789）, P=0.015]. Conclusions High miRNA-223 expression has a significant relation with poor outcome of patients with HCC.

关 键 词：肝癌 MICRORNA microRNA-223 

分 类 号：R735.7[医药卫生—肿瘤]