NEP1-40对缺氧缺血性脑病新生大鼠的Wnt信号通路胞增殖的调控作用  

Role of NEP1-40 in regulation of Wnt signaling pathway and regeneration of neural cells in neonatal rats with hypoxic ischemic encephalopathy

作　　者：王骅[1] 王静成[1] 王永祥[1] 王大新[1] 陶玉平[1] 冯新民[1] 熊传芝 顾加祥[1] 何金山[1] 

机构地区：[1]江苏省苏北人民医院骨科,江苏扬州225000

出　　处：《实用临床医药杂志》2017年第5期1-4,共4页Journal of Clinical Medicine in Practice

基　　金：江苏省扬州市社会发展科技攻关资助项目(YZ2011082);江苏省科技支撑计划-社会发展资助项目(BE2013911);江苏省自然科学基金资助项目(BK20141281)

摘　　要：目的探讨Nogo-A受体拮抗剂NEP1-40对Wnt信号通路和神经细胞增殖的调控作用。方法 40只大鼠被均分为HIBD(缺氧缺血性脑损伤)组和HIBD+NEP1-40组,采用PCR定量、Western blot分析、细胞增殖的免疫组化试验、8-异前列腺素评估等检测分析缺氧缺血性脑病新生大鼠的修复过程中Wnt信号通路中NgR的转录因子调控与神经细胞增殖。结果NEP1-40处理后,c Jun和c-Myc的表达在蛋白水平上调,基因表达水平上调,Ki-67增加,8-异前列腺素无显著变化。结论通过抑制NgR后发现,c-Jun和c-Myc是Wnt通路的主要转录因子,同时脑室下区神经细胞的增殖增加。Objective To explore the role of Nogo-A receptor antagonist NEP1-40 in regulating regeneration of neural cells and related Wnt signaling pathway in neonatal rats with hypoxic ischemic encephalopathy（HIBD）.Methods A total of 40 HIBD rats were divided into HIBD group and HIBD ＋ NEP1-40 group,20 rats in each group.PCR Test,Western Blot Analysis,IHC test for cell proliferation and 8-isoprostane detection were used to evaluate regulation of NgR transcription factors in Wnt signaling pathway and proliferation of neural cells.Results The expressions of c-Jun and cMyc,at the protein level,were up-regulated after treatment with Nogo-A receptor antagonist NEP1-40 for 7 days,and the same change was observed at gene expression and Ki-67.There was no significant change of 8-isoprostane.Conclusion The c-Jun and c-Myc are the main transcription factors in Wnt signaling pathway by inhibition of NgR,and meanwhile the proliferation of nerve cells in subventricular zone increase.

关 键 词：缺氧缺血性脑病 WNT信号通路 NOGO-A NEP1-40 神经细胞增殖 

分 类 号：R742[医药卫生—神经病学与精神病学]