RP-HPLC法同时测定青果中4种成分  

Simultaneous determination of four constituents in Canarii Fructus by RP-HPLC

作　　者：韩雪 卢静华 

机构地区：[1]锦州医科大学,辽宁锦州121000

出　　处：《中成药》2017年第4期773-776,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立RP-HPLC法同时测定青果Canarii Fructus中4种成分的含有量。方法青果甲醇提取液的分析采用Hypersil ODS色谱柱(4.6 mm×200 mm,5μm);以乙腈-0.1%磷酸为流动相,梯度洗脱;体积流量1.0 mL/min;检测波长237 nm;柱温30℃。结果没食子酸、原儿茶酸、绿原酸、表儿茶素分别在0.525~10.500μg/mL(r=0.999 9)、0.700~14.000μg/mL(r=0.999 9)、0.575~11.500μg/mL(r=0.999 9)、0.550~11.000μg/mL(r=0.999 9)范围内线性关系良好,平均加样回收率分别为99.05%(RSD=1.4%)、98.73%(RSD=0.8%)、98.69%(RSD=1.3%)、99.47%(RSD=1.1%)。结论该方法简便、灵敏、可靠,可用于青果的质量控制。AIM To establish a RP-HPLC method for the simultaneous content determination of four constituents in Canarii Fructus. METHODS The analysis of Canarii Fructus methanol extract was performed on a 30 ℃ thermostatic Hypersil ODS column （4.6 mm ×200 mm, 5 μm）, with the mobile phase comprising of acetonitrile- 0. 1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 237 nm. RESULTS Gallic acid, protocatechuic acid, chlorogenic acid and epicatechin displayed good linear relationships within the ranges of 0. 525 - 10. 500 μg/mL （ r = 0. 999 9 ）, 0. 700 - 14. 000 μg/mL （ r = 0. 999 9 ）, 0. 575 - 11. 500 μg/mL （ r = 0. 999 9 ） and 0. 550 - 11. 000 μg/mL （ r = 0. 999 9 ）, whose average recoveries were 99.05% （RSD=1.4%）,98.73% （RSD=0.8%）, 98.69% （RSD=1.3%） and 99.47% （RSD=I.I%）, respectively. CONCLUSION This simple, sensitive and relible method can be used for the quality control of Canarii Fructus.

关 键 词：青果 没食子酸 原儿茶酸 绿原酸 表儿茶素 RP-HPLC 

分 类 号：R284.1[医药卫生—中药学]