草鱼鱼皮胶原蛋白与NIH3T3成纤维细胞的相容性  

The Cytocompatibility of Ctenopharyngodon Idellus Fishskin Collagen with NIH3T3 Fibroblasts

作　　者：余兰[1] 汪海波[2] 陈卓[1] 方成[1] 

机构地区：[1]武汉轻工大学移植工程实验室,武汉430023 [2]武汉轻工大学化学与环境工程学院,武汉430023

出　　处：《中国生物医学工程学报》2017年第2期205-210,共6页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金(21376183);湖北省自然科学基金(2015CFC845)

摘　　要：体外检测草鱼鱼皮酸溶性胶原蛋白(FASC)的细胞相容性。采用MTT实验,检测不同浓度的FASC溶液或FASC涂层胶对NIH3T3成纤维细胞增殖能力的影响(n=6);利用transwell趋化实验,检测不同浓度的FASC溶液对成纤维细胞的趋化作用(n=3);利用transwell侵袭实验,检测不同浓度FASC涂层胶的细胞通透性(n=3)。结果表明,FASC溶液浓度依赖性地促进NIH3T3成纤维细胞增殖,16μg/mL的FASC溶液在48和96 h的增殖率分别为63.7%±7.9%和87.3%±8.7%,较对照组有显著性差异(P<0.001)。NIH3T3细胞能够在FASC涂层胶上生长,各实验组与对照组相比,增殖率均无显著性差异(P>0.05)。趋化实验显示,FASC溶液各浓度梯度组与阴性对照组的趋化指数均有显著性差异(P<0.05),提示FASC对成纤维细胞具有趋化作用。侵袭实验结果显示,成纤维细胞可以通过FASC涂层胶。实验证实,FASC与NIH3T3细胞具有良好的相容性。This study is aimed to test the cytocompatibility of fishskin acid-soluble collagen （FASC） of ctenopharyngodon idellus in vitro. The proliferation rates of NIH3T3 fibroblasts were detected using different concentrations of FASC solutions or coating gums by MTT experiment （n = 6）. The chemotaxis of fibroblasts towards FASC solutions were tested by chemotactic experiments of transwell （n = 3 ）. The cellular permeabilities in different concentrations of FASC coating gums were detected by invasive experiments of transwell （n = 3）. FASC solutions promote proliferation of NIH3T3 fibroblasts and the growth activity of the fibroblasts was dose-dependent on FASC. The proliferation rates of 16μg/mL FASC solution in 48 and 96 h were （63.7 ± 7.9）% and （87.3 ± 8.7）%, that showed significant differences compared with the control group （P 〈 0. 001）. NIH3T3 fibroblasts grew on FASC coating gums without significant differences compared with the control group （P 〉0. 05 ）. The chemotatic indexes showed significant differences between the concentration gradient groups of FASC solutions and the control group （P 〈0. 05） , which indicated that FASC has chemotaxis to fibroblasts. Invasion experiments showed fibroblasts could pass through FASC coating gums. These experiments confirmed that FASC possesses favourable cytocompatibility with NIH3T3 fibroblasts.

关 键 词：胶原蛋白 鱼皮 草鱼 成纤维细胞 细胞相容性 

分 类 号：R318[医药卫生—生物医学工程]