双酚AF胁迫对雄性斑马鱼成鱼甲状腺组织及激素水平的影响  

Effects of BPAF on Thyroid Tissue and Thyroid Hormones of Male Zebrafish( Danio rerio)

作　　者：唐天乐[1,2] 杨洋[2,3] 陈亚文[2,3] 朱小乔[2,3] 唐文浩[2,3] 刁晓平[2,3] 

机构地区：[1]海南医学院热带医学与检验医学院,海口571199 [2]海南大学环境与植物保护学院,海口570228 [3]海南大学海口市环境毒理学重点实验室,海口570228

出　　处：《生态毒理学报》2017年第1期127-133,共7页Asian Journal of Ecotoxicology

基　　金：海南省科协青年科技人才学术创新计划项目(No.201513);海南医学院科研培育基金(No.HY2014-013)

摘　　要：为评价双酚AF(BPAF)的甲状腺毒性,以雄性斑马鱼成鱼为受试模式生物,采用半静态染毒的方式对雄性斑马鱼成鱼染毒14 d(0、5、50和500μg·L^(-1)),分别设置7 d、14 d 2个时间节点,通过酶联免疫法(ELISA)分别检测斑马鱼血浆中总三碘甲腺原氨酸(TT3)、总甲状腺素(TT4)、游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)的含量水平,并对14 d暴露后斑马鱼甲状腺进行组织学检测分析。结果显示随着暴露浓度的提高,出现甲状腺滤泡上皮细胞增生,滤泡组织变形明显,细胞胶质缺失等病理学变化。雄性斑马鱼在BPAF暴露情况下,血浆中TT3、TT4、FT3、FT4含量随着暴露浓度的提升和时间的延长出现显著上升,呈现出甲状腺功能亢奋的干扰效应。In order to evaluate the endocrine toxicity of BPAF,the male zebrafish（Danio rerio）was used as a target animal and exposed to 0,5,50 and 500μg·L^-1BPAF for 14 days by way of semi static exposure.In this study,the concentrations of total 3,3＇,5-triiodothyronine（TT3）,total 3,5,3＇,5＇-tetraiodothyronine（TT4）,free 3,3＇,5-triiodothyronine（FT3）,free 3,5,3＇,5＇-tetraiodothyronine（FT4）in plasma of zebrafish were detected by ELISA after exposure for 7 days and 14 days.The thyroid gland of zebrafish was analyzed by histological techniques after exposure for 14 days.The results showed increased number of epithelial cells,cell colloid deficiency and obvious follicle tissue deformation in the thyroid with the increase of BPAF exposure concentration.The concentration of thyroid hormones（THs）in plasma of male zebrafish increased significantly with the increase of BPAF exposure concentration and time.It showed the interference effect of hyperthyroidism.

关 键 词：双酚AF 斑马鱼 甲状腺组织 甲状腺激素水平 

分 类 号：X171.5[环境科学与工程—环境科学]