从江香猪DRD1基因多态及其生物信息学分析  

Analysis of the Polymorphisms and Bioinformatics of DRD1 Gene in Congjiang Xiang Pig

作　　者：白优[1] 张勇[1] 陈祥[1,2] 李俊 张雄[1] 何琦[1] 杨红[1] 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州大学动物科学学院,贵阳550025 [2]贵州省生猪健康养殖工程技术研究中心,贵阳550025

出　　处：《基因组学与应用生物学》2017年第2期570-574,共5页Genomics and Applied Biology

基　　金：贵州省工程中心建设项目(黔科合农C字[2011]4022号);贵州大学引进人才科研项目提高香猪产仔数及仔猪成活率关键技术研究与应用”;贵大人基合字2013(21)号共同资助

摘　　要：为揭示猪DRD1基因多态性,本研究以从江香猪为研究对象,以外三元(杜×长×大)杂交猪及贵州宗地花猪为对照,采用DNA池和直接测序技术,筛查DRD1基因所有外显子区SNPs位点;利用生物信息学软件估算等位基因频率并预测SNPs位点对m RNA二级结构的影响。结果表明,在DRD1基因中共筛查到1个SNPs位点,位于5'UTR区为T-182G;从等位基因频率角度分析,从江香猪与贵州宗地花猪估算得到的等位基因频率较一致,与外三元(杜×长×大)杂交猪却存在较大差异;利用RNA secondary structure prediction软件预测,发现mTo reveal the polymorphism ofDRD1 gene object, and regarded the crossbred growing pig （Duroc in pig, we selected the Congjiang Xiang pig as the research × Landrace ×Big White） and Guizhou Zongdi Hua pig as the contrast. DNA pool and direct sequencing technology were used to screen SNPs sites in all exon sites of DRD1 gene. Bioinformatics software was used to estimate allele frequencies and to predict the effect of the SNPs on mRNA secondary structure. The results showed that there was a SNPs site in DRD1 gene, which was T-182G locatng in 5＇ UTR region. From the perspective of allele frequencies, the estimated allele gene frequency of Congjiang Xiang pig was same with that of the Guizhou zongdi Hua pig, but it was quite different from that of the crossbred growing pigs. The result of RNA secondary structure prediction indicated that the secondary structure of mRNA was significantly changed.

关 键 词：从江香猪 DRD1基因 多态性 生物信息学 

分 类 号：S828[农业科学—畜牧学]