富氢生理盐水对大鼠痛风性关节炎的保护作用  

Study on the effect of hydrogen rich saline on gout arthritis in rats

作　　者：招友 樊彦伟[2] 马广斌[2] 严冬雪[2] 

机构地区：[1]河池市人民医院骨科,广西河池547000 [2]桂林医学院附属医院骨科,广西桂林541001

出　　处：《广东医学》2017年第5期672-674,共3页Guangdong Medical Journal

摘　　要：目的探讨富氢生理盐水对大鼠痛风性关节炎(GA)的保护作用。方法雄性Wistar大鼠40只,随机分为4组:正常对照组、模型组、秋水仙碱组、富氢盐水组,富氢盐水组予10 mL/kg富氢盐水,秋水仙碱组予0.3 mg/kg秋水仙碱,正常对照组、模型组给予同体积的吐温-生理盐水,各组均灌胃给药2次/d,连续给药4周,在最后1次灌胃后采用尿酸钠右踝关节腔注射法进行大鼠GA的造模。于造模前后分别测量大鼠受试关节周径,白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的含量,并取受试关节软组织行病理检查,结合大鼠的行为学表现对治疗效果进行评价。结果与模型组比较,富氢盐水组、秋水仙碱组大鼠步态有明显改善(P<0.05),受试关节周径明显缩小(P<0.01),血清中IL-1β、TNF-α表达水平显著降低(P<0.01),右踝关节滑膜炎症反应显著降低。结论富氢生理盐水能减轻GA大鼠炎症反应,对大鼠GA具有一定的疗效。Objective To investigate the protective effects of hydrogen rich saline on acute gouty arthritis model in rats. Methods Forty rats were randomly divided into four groups, control group, model group, hydrogen rich saline group and eolchicine group. In eolchicine group, 0.3 mg/kg colchicine were given with lavage; l0 mL hydrogen rich sa- line were also given in hydrogen rich saline group continuously for 4 weeks. The control group and the model group were given with the same volume of Twain - saline. Acute gouty arthritis model was established at the last time after pretreat- ment. The acute gouty arthritis models were established on the last time after intragastric administration, by the method of intraarticular injection of sodium urate. The subject joint circumference, interleukin - 113 （ IL - 1β） and tumor necrosis factor -α （ TNF - α） were measured before and after model establishment. The joint soft tissues were collected for histo- pathology examination. Besides, combine the behaviors of rats to evaluate the therapeutic effect. Results Compared to the model group, significant improvement in joint circumference, gait, serum IL - 1β and TNF -α and the inflammatory reaction of subject ankle joint were revealed in colchicine group and hydrogen rich saline group colehicine group. Conclusion Hydrogen rich saline can reduce the inflammatory reaction and also effectively inhibit the gout arthritis in rats.

关 键 词：痛风性关节炎 富氢生理盐水 白细胞介素-1Β 肿瘤坏死因子-Α 

分 类 号：R-332[医药卫生]