急性感染期猪伪狂犬病病毒部分基因组染色质状态  

Chromatin state of some genome segment of pseudorabies virus during lytic infection

作　　者：张玉娟[1] 韩莎莎[1] 时庆贺 石昂 杨利[1] 常洪涛[1] 赵军[1] 王川庆[1] 陈陆[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国兽医学报》2017年第4期585-591,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31272567);河南省高校科技创新团队支持计划资助项目(14HASTIT022);河南省高校科技创新团队支持计划资助项目(14IRTSTHN015)

摘　　要：为探究急性感染期猪伪狂犬病病毒(PRV)在宿主细胞内基因组染色质的状态,首先建立PRV实时荧光定量PCR(qPCR)方法并测定0.1 MOI PRV感染Neuro-2a细胞后病毒基因组的复制动态,然后收集PRV感染Neuro-2a细胞的样品进行染色质免疫共沉淀试验(CHIP),并通过qPCR测定病毒部分DNA与组蛋白H3结合形成的染色质状态。结果显示,PRV急性感染Neuro-2a细胞后,部分基因组以染色质结构存在,并与病毒复制有一定相关性。本研究成功建立用于研究PRV基因组染色质状态的CHIP试验方法,为PRV急性感染期表观遗传学研究提供依据。In order to determine the chromatin status lytic infection,firstly the PRV real-time fluorescent of pseudorabies virus（PRV）in host cell during quantitative PCR（qPCR）was established and used to determine the multiplication of virus genome in Neuro-2a cell infected with 0.1 MOI PRV. Then the Neuro-2a cell infected with PRV was collected for chromatin immunoprecipitation （CHIP）. The chromatin status of virus genome associated with histone H3 was detected by real- time PCR. The results showed that some genome segment of PRV is present as chromationl, which plays a regulatory role during PRV lytic infection. In this study, the CHIP method for detecting PRV chromatin state was successfully established. The results laid the foundation for the further studies on PRV epigenetics during lytic infection.

关 键 词：猪伪狂犬病病毒 急性感染 染色质状态 染色质免疫共沉淀试验(CHIP) 

分 类 号：S855.3[农业科学—临床兽医学]