Lgr5、β-catenin在卵巢上皮性癌组织中的表达及对SKOV3细胞增殖迁移的影响  

Expressions of Lgr5 and β-catenin in epithelial ovarian cancer tissue and the effect on cell proliferation and migration of SKOV3 cells

作　　者：张展[1] 朱海[1] 宋华[1] 王金铭[1] 

机构地区：[1]郑州大学第三附属医院妇产科,郑州450052

出　　处：《郑州大学学报（医学版）》2017年第2期138-142,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技厅基础与前沿项目152300410190

摘　　要：目的:探讨G蛋白偶联受体家族Lgr5和β-catenin在卵巢上皮性癌中的表达及对SKOV3细胞增殖和迁移能力的影响。方法:收集卵巢正常、良性肿瘤及上皮性癌组织标本各30例,采用免疫组化法检测Lgr5和β-catenin蛋白的表达情况。用Lgr5 shRNA、NC shRNA转染卵巢癌SKOV3细胞,同时以未转染细胞作空白对照,分别采用Western blot和实时荧光定量PCR法检测3组细胞中lgr5、β-catenin蛋白和mRNA的表达水平,分别采用CCK-8和划痕实验检测3组细胞的增殖和迁移能力。结果:卵巢上皮性癌组织中Lgr5、β-catenin阳性表达率分别为63.3%(19/30)、83.3%(25/30),二者表达水平均较良性肿瘤、正常卵巢组织升高(P<0.05),且两者表达呈正关联(rp=0.373,P=0.028)。与空白对照组、NC shRNA转染组比较,Lgr5 shRNA转染组SKOV3细胞中Lgr5、β-catenin蛋白和mRNA的表达均显著降低(P<0.05),细胞的增殖能力和迁移能力显著降低(P<0.05)。结论:Lgr5可能通过增强Wnt/β-catenin信号通路参与卵巢癌细胞增殖、迁移能力的调控。Aim： To investigate the expressions of G protein coupled receptor Lgr5 and β-catenin in ovarian epithelial cancer tissue and the effect on the cell proliferation and migration of SKOV3 cells. Methods： The expressions of Lgr5 andβ-catenin protein in tissue from 30 cases of ovarian cancer and 30 cases of ovarian benign tumor,as well as 30 normal ovarian tissue samples were detected by immunohistochemistry. The SKOV3 cells were transfected with Lgr5 shRNA,and those transfected with NC shRNA and without transfection were the control. The protein and mRNA expressions of Lgr5 and β-catenin in SKOV3 cells were detected by Western blot and qRT-PCR,respectively; cell proliferation was detected by CCK-8 assay and the cell migration was detected by scratch assay. Results： The positive rates of Lgr5 and β-catenin in epithelial ovarian cancer tissue were 63. 3%（ 19 /30） and 83. 3%（ 25 /30）,which were higher when compared with the normal ovarian tissue and ovarian benign tumor tissue（ P〈0. 05）. There was positive correlation between the expressions of Lgr5 andβ-catenin in epithelial ovarian cancer tissue（ rp= 0. 373,P = 0. 028）. Compared with the two control groups,the protein and mRNA expressions of Lgr5 and β-catenin in SKOV3 cells transfected with Lgr5 shRNA were significantly lower（ P〈0. 05）,and proliferation and migration capacity were significantly lower（ P〈0. 05）. Conclusion： Lgr5 may regulate the proliferation and migration of SKOV3 cells by enhancing the signal pathway of Wnt / β-catenin.

关 键 词：LGR5 WNT/Β-CATENIN信号通路 卵巢上皮性癌 细胞增殖 细胞迁移 SKOV3细胞 

分 类 号：R737.31[医药卫生—肿瘤]