牛角瓜果提取物的抑菌活性研究  

Antifungal and antimicrobial activity of fruit extracts from Calotropis gigantea

作　　者：杨军辉[1] 杨彩霞[2] 高非[1] 王祝年[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [2]西北师范大学化学化工学院

出　　处：《中国消毒学杂志》2017年第3期218-220,共3页Chinese Journal of Disinfection

基　　金：中央级公益性科研院所基本科研业务费用专项(1630032015027);海南省重点研发计划(ZDYE2016173)

摘　　要：目的研究牛角瓜果提取物体外抑菌活性,为实际应用提供科学依据。方法采用纸片扩散法,对牛角瓜果的石油醚、乙酸乙酯、氯仿和甲醇等4种溶剂提取物体外抑菌活性进行实验室研究。结果牛角瓜果的石油醚提取物浓度为100 mg/ml,对13种试验菌中的8种菌(含真菌和细菌)的抑菌环直径为7.12~12.83 mm。牛角瓜果乙酸乙酯提取物抑菌作用较石油醚提取物弱,其他试剂的提取物基本无抑菌作用。结论牛角瓜果的石油醚提取物具有一定抑菌作用,但总体上抑菌效果较弱。Objective To evaluate the antifungal and antimicrobial activity of the fruit extracts from Calotropis gigantea in vitro. Methods The fruit was extracted by Soxhlet extraction method with four different solvents, including petroleum ether, ethyl acetate, chloroform and methyl alcohol. The antifungal and antimicrobial activities were evaluated using disc agar diffusion method. Results The petroleum ether fruit extracts exhibited good antimicrobial activities against eight of thirteen tested fungi and bacteria,with the inhibitory zone diameters from 7.12 - 12.83 mm at a concentration of 100 mg/ml. And other fruit extracts show no activities. Conclusion The petroleum ether fruit extracts presented a generally poor antifungal and antimicrobial activity.

关 键 词：牛角瓜 果提取物 抑菌活性 纸片琼脂扩散法 

分 类 号：R187.1[医药卫生—流行病学]