MicroRNA-10b对人胃癌SGC-7901细胞增殖、迁移和侵袭能力的影响  

Effect of MicroRNA-10b on the proliferation, migration and invasion of gastric cancer cells SGC-7901

作　　者：赵林[1] 张伟[2] 张玉扬[1] 

机构地区：[1]抚顺矿务局总医院肿瘤内科,辽宁抚顺113008 [2]抚顺矿务局总医院病理科,辽宁抚顺113008

出　　处：《解剖科学进展》2017年第2期154-157,共4页Progress of Anatomical Sciences

摘　　要：目的探讨Micro RNA-10b(miR-10b)对人胃癌SGC-7901细胞增殖、迁移和侵袭的影响。方法应用Real-time PCR方法检测21例胃癌组织和癌旁正常组织中miR-10b的表达水平,应用Lipofectamine?LTX试剂将化学合成的miR-10b mimics和miR-10b inhibitor转染至胃癌SGC-7901细胞,采用MTT方法检测SGC-7901细胞增殖能力的变化,Transwell小室法检测SGC-7901细胞迁移和侵袭能力的变化;应用Western Blot方法检测P21蛋白表达水平。结果与正常组织相比,miR-10b在胃癌组织中的表达水平显著增高。与对照组相比,miR-10b表达沉默能够显著抑制人胃癌SGC-7901细胞的增殖、迁移和侵袭能力,显著增加P21的蛋白表达水平。miR-10b过表达的作用与之相反。结论 MiR-10b沉默抑制胃癌SGC-7901细胞的增殖、迁移和侵袭可能与上调P21蛋白表达相关。Objective To study the effects of Micro RNA-10 b on the proliferation, migration and invasion of gastric cancer cells SGC-7901. Method The mRNA expression of miR-10 b was detected by real-time PCR in 21 cases of gastric cancer tissues and normal tissues. Lipofectamine？ LTX was applied to transfecting gastric cancer cells SGC-7901 with miR-10 b mimics or inhibitor, and then MTT was applied to detect the viability of gastric cancer cells SGC-7901. Transwell chamber assay was used to measure the migration and invasion of gastric cancer cells SGC-7901. The expression of P21 protein was detected by Western blot. Results The expression level of miR-10 b was significantly higher in gastric cancer tissues than in normal tissues. MiR-10 b silence significantly inhibited the proliferation, migration and invasion ability of gastric cancer cells SGC-7901 and obviously increased the expression level of P21 protein, but with opposite role for miR-10 b overexpression. Conclusion The inhibition effect of MiR-10 b silencing on the proliferation, migration and invasion of gastric cancer cells SGC-7901 might be related to the upregulation of P21.

关 键 词：MIR-10B 人胃癌SGC-7901细胞 P21 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]