miR-124靶向Sp1对人骨肉瘤MG-63细胞增殖的调控  

miR-124 regulates the proliferation of human osteosarcoma MG-63 cells by targeting Sp1

作　　者：戴颖[1] 王强[1] 

机构地区：[1]沈阳市骨科医院骨感染科,辽宁沈阳110044

出　　处：《解剖科学进展》2017年第2期167-170,共4页Progress of Anatomical Sciences

基　　金：辽宁省教育厅科研基金(L2010687)

摘　　要：目的探讨miR-124是否能够通过靶向抑制Sp1基因调控人骨肉瘤MG-63细胞的增殖。方法利用荧光素酶报告基因实验验证Sp1基因是否为miR-124的靶基因,应用阳离子脂质体LipofectamineTM2000将miR-124 mimics或inhibitor转染至MG-63细胞,利用real-time PCR和Western blotting分析转染后MG-63细胞Sp1 mRNA和蛋白表达的变化,通过MTT法分析转染后MG-63细胞增殖水平变化。结果荧光素酶报告基因实验证实Sp1基因为miR-124的靶基因。与对照组相比,转染miR-124 mimics后,MG-63细胞Sp1 mRNA与蛋白的表达均显著降低,增殖能力明显下降(P<0.01);与对照组相比,转染miR-124 inhibitor后,MG-63细胞Sp1mRNA与蛋白的表达均显著升高,增殖能力明显升高(P<0.01)。结论 miR-124抑制人骨肉瘤MG-63细胞增殖可能与靶向Sp1基因相关。Objective To investigate whether miR-124 regulates the proliferation of human osteosarcoma cell line MG-63 by targeting Sp1 gene.Methods Luciferase reporter gene assay was used to detect whether Sp1 was target gene of miR-124, miR-124 mimics or inhibitor was transfected into MG-63 cells by lipofectamine package, then realtime PCR and Western blot were used to evaluate the expression of Sp1 mRNA and protein, respectively, the proliferation of MG-63 cells was analyzed by MTT assay.Results The luciferase reporter gene assay showed that Sp1 was target gene of miR-124; compared with untreated group, the m RNA and protein expression levels of Sp1 and the viability of MG-63 cells were decreased significantly after miR-124 mimics was transfected into MG-63 cells（P〈0.01）, but increased significantly after miR-124 inhibitor was transfected into MG-63 cells. Conclusion The inhibition of miR-124 on the proliferation of human osteosarcoma MG-63 cells might be related to targeting Sp1 gene.

关 键 词：miR-124 骨肉瘤 SP1 增殖 MG-63细胞 

分 类 号：R738.1[医药卫生—肿瘤]