异甘草酸镁对小鼠放射性肺纤维化的改善作用及其机制的初步研究  

Preliminary study on effect and mechanism of MgIG in improvement of pulmonary fibrosis induced by radiation in mice

作　　者：张攀[1] 刘涛[1] 王芳芹 阳群芳[1] 陈晓红[1] 

机构地区：[1]第三军医大学药学系药理学教研室,重庆400038

出　　处：《中国药理学通报》2017年第2期201-206,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No81472912)

摘　　要：目的探讨异甘草酸镁(magnesium isoglycyrrhizinate,MgIG)对小鼠放射性肺纤维化的影响及其机制。方法 50只♀C57BL/6小鼠随机分为对照组(Control组)、辐照组(RT组)、异甘草酸镁组(MgIG组)、异甘草酸镁治疗组(RT+MgIG组)和地塞米松治疗组(RT+DXM组),每组10只。除Control组和MgIG组外,其余各组小鼠均给予15Gy^(60)Coγ射线全胸单次照射。各组小鼠于辐照前2 h及辐照后每日给药:MgIG组和RT+MgIG组腹腔注射MgIG(100 mg·kg^(-1));Control组和RT组腹腔注射生理盐水(20 m L·kg^(-1));RT+DXM组腹腔注射DXM(0.5 mg·kg^(-1))。辐照后12周取材,行HE染色及Masson染色观察小鼠肺泡炎和肺纤维程度,免疫组化检测肺组织Ⅰ型胶原(CollagenⅠ)、Ⅲ型胶原(CollagenⅢ)和转化生长因子-β1(TGF-β1)蛋白表达。结果 RT组小鼠肺泡炎和肺纤维化程度及CollagenⅠ、CollagenⅢ、TGF-β1、p-Smad2、p-Smad3蛋白表达较Control组明显增强(P<0.05),而RT+MgIG组与RT+DXM组肺泡炎、肺纤维化程度及蛋白表达相较于RT组明显降低(P<0.05)。结论异甘草酸镁可有效改善小鼠放射性肺纤维化,其机制可能与调控TGF-β信号通路有关。Aim To investigate the effect of magnesi-um isoglycyrrhizinate （MgIG）on radiation -induced pulmonary fibrosis in mice and the mechanism.Meth-ods Fifty female C57BL/6 mice were randomly divid-ed into control group,irradiation （RT）group,MgIG group,RT ＋MgIG group and RT ＋dexamethasone （DXM）group,with 1 0 mice in each group.Except for control group and MgIG group,the remaining mice were given a single 1 5Gy 60 Co γray on whole lung. The mice in each group were administered 2 h before irradiation and each day after irradiation：MgIG group and RT ＋MgIG group were administered with MgIG （1 00 mg·kg^-1 ）by intraperitoneal injection;control group and RT group were administered with normal sa-line （20 mL·kg^-1 ）by intraperitoneal injection;RT＋DXMgroup was administered with DXM（0.5 mg· kg^-1 ）by intraperitoneal injection.After 1 2 weeks,the mice were sacrificed and lung tissues were taken out. The degree of alveolitis and pulmonary fibrosis were observed by HE staining and Masson staining.The ex-pressions of type Ⅰ collagen,type Ⅲ collagen and TGF-β1 protein were detected by immunohistochem-isty.Results The alveolitis,pulmonary fibrosis and expressions of type Ⅰ collagen,type Ⅲ collagen, TGF-β1 ,p-Smad2,p-Smad3 increased significantly in RT group compared with control group （P 〈0.05 ）, and were significantly lower in RT ＋MgIG group and RT ＋DXMgroup than those in RT group（P 〈0.05）. Conclusion MgIG can improve radiation-induced pulmonary fibrosis in mouse lung tissue,and its mech-anism may be related to the influence of MgIG on TGF-βsignaling pathway.

关 键 词：异甘草酸镁 放射性肺纤维化 Ⅰ型胶原 Ⅲ型胶原 转化生长因子β/Smad信号通路 小鼠 

分 类 号：R-332[医药卫生] R284.1