蒽贝素对脂多糖诱导小鼠心脏炎症损伤的影响  

Cardioprotection and mechanism of embelin in sepsis mice model

作　　者：钱香[1] 闫涛[1] 齐艳[1] 陈莹莹[2] 

机构地区：[1]杭州市中医院,浙江杭州310007 [2]浙江大学医学院

出　　处：《预防医学》2017年第4期355-359,共5页CHINA PREVENTIVE MEDICINE JOURNAL

摘　　要：目的研究蒽贝素对脂多糖(LPS)诱导小鼠心肌损伤的影响及其作用机制。方法从80只C57BL/6J(B6)雄性小鼠中随机抽取16只作为正常对照组,腹腔注射生理盐水10 mg/kg;其余64只腹腔注射10 mg/kg LPS,等分为脓毒症阳性对照组和低、中、高浓度治疗组4组,每组16只,治疗组小鼠再分别腹腔注射5、10、20 mg/kg蒽贝素。采用双位点酶免法测定血清中心肌肌钙蛋白I(c Tn I)水平,免疫组化法检测心肌PPAR-γ和TNF-α水平,蛋白免疫印迹试验(WB)检测NF-κB水平。结果正常对照组、中浓度治疗组、高浓度治疗组小鼠血清c Tn I水平、细胞核内NF-κB蛋白灰度比值和心肌细胞TNF-α黄染率均低于脓毒症阳性对照组(均P<0.05);脓毒症阳性对照组与正常对照组小鼠心肌细胞PPAR-γ黄染率差异无统计学意义(P>0.05),各治疗组小鼠心肌细胞PPAR-γ黄染率均升高(P<0.01)。结论蒽贝素可剂量依赖性地改善LPS诱导的小鼠心肌损伤,其机制可能为上调PPAR-γ表达、抑制NF-κB活化和减少TNF-α合成。Objective To observe the effect of embelin on the cardiac injury in sepsis mice model,and to explore whether PPAR-participates in the mechanism of cardioprotection of embelin. Methods Male mice were randomly divided into five groups. Control group were received PBS i. p. injection. Sepsis model group were received LPS（ 10 mg/kg） i. p.injection. Embelin treatment groups were received LPS（ 10 mg/kg） i. p. injection,and after 30 min different doses of embelin were given（ 5,10,and 20 mg/kg,i. p.）. Results The serum c Tn I level and TNF-α、NF-κB protein in low dose embelin（ 5 mg/kg） treatment mice was not different with that of the sepsis mice. But the serum c Tn I level and TNF-α、NF-κB protein in middle and high doses embelin（ 10,20 mg/kg） treatment mice was lower than that of sepsis mice,and the expression of PPAR-γ was higher（ P 〈 0. 05）. Conclusion Embelin could protect against sepsis-induced cardiac injury. The mechanism might be involved in the upregulation of PPAR-γ protein,inhibition of NF-κB activation,and reduction of TNF-α level.

关 键 词：蒽贝素 脓毒症 NF-ΚB TNF-Α PPAR-Γ 

分 类 号：R54[医药卫生—心血管疾病]