Detection and Analysis on StaphylococcalEnterotoxins in Raw Cow Milk from DifferentRegions of Jinan, China  

作　　者：Congchao ZHANG Jinglong LI Wenbo WANG Lei LIU Xiaohong GU 

机构地区：[1]School of Bioengineering, Qilu University of Technology, Jinan 250353, China [2]Institute of Agricultural Quality Standards and Testing Technology, shandongAcademy of Agricultural Sciences, Jinan 250100,China [3]Longrun Biological Technology Co., Ltd., Beiiincl 102200, China [4]Shandong Center of Crop Germplasm Resources, Jinan 250100, China

出　　处：《Agricultural Science & Technology》2017年第3期465-468,共4页农业科学与技术（英文版）

摘　　要：[Objective] This study was conducted to investigate the pollution caused by staphylococcal enterotoxins(SE) in raw milk and the safety of dairy products in Jinan, and to provide a scientific basis for food safety risk analysis. [Method] A total of 130 raw milk samples were collected from different regions of Jinan, and detected for Staphylococcus aureus by referring to GB4789.10-2010. Then, enzyme-linked immunosorbent assay was performed to detect whether the S.aureus strains produced enterotoxins, and the enterotoxin type was identified using colloidal gold-based immunochromatographic test strips. [Result] Fiftyseven of the raw milk samples were polluted by S.aureus, so the detection rate of S.aureus was 43.85%; and 11 of the strains produced enterotoxins. Among the 11 enterotoxin-producing strains, seven produced SEB, only one produced SEC, and the SE type of other three strains was not identified. [Conclusion] Enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic test strips can be used in combination to rapidly detect staphylococcal enterotoxins and identify enterotoxin type, although there are some limitations. SEB is the main type of staphylococcal enterotoxin causing pollution in milk of Shandong Province.[Objective] This study was conducted to investigate the pollution caused by staphylococcal enterotoxins(SE) in raw milk and the safety of dairy products in Jinan, and to provide a scientific basis for food safety risk analysis. [Method] A total of 130 raw milk samples were collected from different regions of Jinan, and detected for Staphylococcus aureus by referring to GB4789.10-2010. Then, enzyme-linked immunosorbent assay was performed to detect whether the S.aureus strains produced enterotoxins, and the enterotoxin type was identified using colloidal gold-based immunochromatographic test strips. [Result] Fiftyseven of the raw milk samples were polluted by S.aureus, so the detection rate of S.aureus was 43.85%; and 11 of the strains produced enterotoxins. Among the 11 enterotoxin-producing strains, seven produced SEB, only one produced SEC, and the SE type of other three strains was not identified. [Conclusion] Enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic test strips can be used in combination to rapidly detect staphylococcal enterotoxins and identify enterotoxin type, although there are some limitations. SEB is the main type of staphylococcal enterotoxin causing pollution in milk of Shandong Province.

关 键 词：RAW MILK STAPHYLOCOCCUS AUREUS ENTEROTOXIN 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]