响应面法优化单酶复配酶解辐照玉米秸秆的研究  

Optimization of Enzyme Complex Formulation of Irradiation Corn Straw by Response Surface Method

作　　者：滕彦淇 胡秋龙[2,3] 苏小军[2,3] 李会东[4] 金元昌[1,5] TENG Yan-qi Hid Qiu-long SU Xiao-jun JIN Yuan-chang et al(College of Life Science, Hunan University of Science and Technology ,Xiangtan, Hunan 411201 Hunan Engineering Laboratory for Alcohol Fuels from Biomass, Hunan Agricultural University,Changsha, Hunan 410128 Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128 5. Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-polluted Soils,College of Hnnan Provinee,Xiangtan,Hunan 411201)

机构地区：[1]湖南科技大学生命科学学院,湖南湘潭411201 [2]湖南农业大学生物质醇类燃料湖南省工程实验室,湖南长沙410128 [3]湖南农业大学湖南省植物功能成分利用协同创新中心,湖南长沙410128 [4]内蒙古工业大学,内蒙古呼和浩特010051 [5]重金属污染土壤生态修复与安全利用湖南省高校重点实验室,湖南湘潭411201

出　　处：《安徽农业科学》2017年第10期1-4,47,共5页Journal of Anhui Agricultural Sciences

基　　金：湖南省科技重大专项(2015NK1001-4);湖南省教育厅重点项目(15A067);生物工程专业省级校企合作人才培养示范基地项目(G145813)

摘　　要：[目的]优化单酶复配酶解纤维素的条件。[方法]以经1 200 kGy^(60)Coγ-射线辐照处理过的玉米秸秆为原料,采用响应面分析法,对外切葡聚糖酶、内切葡聚糖酶、β-葡萄糖苷酶、木聚糖酶等酶进行优化。[结果]4个因素对酶解产还原糖的影响主次顺序依次为木聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶和内切葡聚糖酶。得到最优酶添加量为外切葡聚糖酶1.07 U/g、内切葡聚糖酶31.53 U/g、β-葡萄糖苷酶20.81 U/g和木聚糖酶81.96 U/g。在上述条件下,试验验证还原糖产量372.624 mg/g与预测值能够很好地吻合。[结论]该中心组合设计响应面优化单酶复配酶解辐照玉米秸秆的方法可靠,可为单酶复配提供参考。[ Objective] To study the optimal ratio of enzyme complex formulation. [ Method] 1 200 kGy ^60Co γ-ray irradiation corn straw as raw material,the response surface methodology was applied for analyzing the cellobiohydrolase,the endoglucanase, the β-glucanase and the xylanase. [ Result] The reducing sugar content was extremely significantly by xylanase dosage but not significantly impacted by cellobiohydrolase, β-glucanase and endoglucanase. The optimum conditions for supplemental levels were found to be hydrolysis at cellobiohydrolase ratio of 1.07 U/g ,endoglucanase ratio of 31.53 U/g, β-glucanase ratio of 20.81 U/g and xylanase ratio of 81.96 U/g. The reducing sugar content under these conditions was 372.624 mg/g than before optimization and basically according with the model prediction value. [ Conclusion] The optimization of enzyme complex formulation of irradiation corn straw by central composite design and response surface method is reliable, and it can provide reference for the complex of single enzyme.

关 键 词：γ-射线辐照 响应面法 酶解 葡萄糖 

分 类 号：S216[农业科学—农业机械化工程]