野生大豆与栽培大豆杂交F_1代ISSR多态性分析  

ISSR Polymorphism Analysis of F_1 Hybrids of Glycine soja and Glycine max

作　　者：熊振宇[1] 李海波[1] 余爽[1] XIONG Zhen-yu LI Hai-bo YU Shuang(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan,Hubei 43000)

机构地区：[1]武汉轻工大学生物与制药工程学院,湖北武汉430000

出　　处：《安徽农业科学》2017年第10期137-139,209,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探究野生大豆与栽培大豆之间遗传多态性的差异,以期为大豆杂交育种的亲本选配提供理论依据。[方法]用20对ISSR引物对野生大豆与栽培大豆及其10份杂种F_1材料进行遗传多样性分析,探讨野生大豆与栽培大豆之间的遗传关系。[结果]此次试验共扩增出167条带,其中121条为多态性带,多态率达72.45%;杂交F_1代材料与其母本中黄19及父本ZYD04350间的平均遗传相似系数分别为0.65和0.47。[结论]栽培大豆与野生大豆杂交F_1代材料间存在着丰富的变异,杂交F_1代从母本获得的遗传物质多于父本。[ Objective ] To sudy the difference of genetic polymorphism between Glycine soja and Glycine max, so as to provide theory evidence for C.lycine max crossbreeding. [ Method ] Twenty primers were used for ISSR analysis on Glycine soja, Glycine max and 10 F1 hybrids, to study the genetic relationship of Glycine soja and Glycine max. [ Result] Total 167 bands were amplified in the PCR reactions. Among them 121 were polymorphie bands and the polymorphic rate was 72.45%. The average genetic coefficients between Zhonghuang 19 and F1 progenies ,ZYD04350 and F1 progenies were 0.65 and 0.47, respectively. [ Conclusion ] There was abundant genetic variation among F1 progenies derived from eressing between Glycine soja and Glycine max, genetic material of F1 hybrids obtained from maternal was more than male parents.

关 键 词：野生大豆 栽培大豆 ISSR 多态性 

分 类 号：S565.1[农业科学—作物学]