束缚应激对大鼠咬肌区域痛觉敏感及三叉神经脊束核神经元和星形胶质细胞的影响  

The effect of restraint stress on masseter mechanical hyperalgesia and the activity of neurons and astrocytes in the spinal trigeminal nucleus caudalis in rats

作　　者：林文青[1] 张旻 赵寅华[1] 李强[1] 王舰[2] 陈永进[1] 

机构地区：[1]第四军医大学口腔医学院急诊与综合临床科军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病国际联合研究中心,西安710032 [2]成都军区总医院心血管外科,610083

出　　处：《中华口腔医学杂志》2017年第4期254-258,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81271167、81500851）;陕西省科技统筹创新工程计划（2016KTCQ03-05）

摘　　要：目的研究束缚应激对大鼠咬肌区域痛觉敏感及三叉神经脊束核尾侧亚核（spinal trigeminal nucleus caudalis，Vc）中神经元、星形胶质细胞活性的影响，为进一步探究心理应激与15颌面痛的相关关系提供参考。方法将大鼠分为对照组和应激1、3、5、7、9、11、14d组，每组10只；各应激组束缚应激相应时间后评价模型有效性；建模成功后检测大鼠咬肌区域机械性痛觉阈值的变化，免疫荧光技术检测Vc中c-fos蛋白、胶质细胞原纤维酸性蛋白（glial fibrillary acidic protein，GFAP）的表达。结果束缚应激能引起大鼠咬肌区域机械性痛觉阈值下降，其中应激7、9、11、14d组大鼠咬肌区域机械性痛觉阈值[（0．071±0．011）、（0．059±0．020）、（0．052±0．011）、（0．033±0．011）州均显著小于对照组[（0．120±0．025）NI（P〈0．05）。应激1d组大鼠c-fos阳性神经元数[（1050±129）个／mm^2]比对照组[（550±129）个／mm^2]显著升高（P〈0．05），持续至应激1周后逐渐恢复至对照组水平；应激7、9、11、14d组大鼠GFAP表达（4．3±1．0、4．5±0．6、4．6±0．5、4．8±1.3）均较对照组（2．0±0．8）显著升高（P〈0．05）。结论慢性束缚应激可导致大鼠咬肌区域机械性痛觉阈值下降，并活化Vc内神经元与星形胶质细胞，提示vc内神经元与星形胶质细胞的活化可能在束缚应激致大鼠咬肌区域痛觉敏感中发挥重要作用。Objective To investigate the effect of restraint stress on masseter mechanical hyperalgesia and the activity of neurons and astrocytes in the spinal trigeminal nucleus caudalis （Vc）. Methods The animals were randomly divided into the control group, 1-, 3-, 5-, 7-, 9-, 11- and 14-day stress groups, with 10 rats in each group. The body weight increase and behavior tests were used to testify the animal model. The mechanical sensitivity of masseter of the rat before and after the stress was measured with Von Frey filaments. Histological examinations were used to evaluate the expression of neuronal c-fos and astrocytic glial fihrillary acidic protein （GFAP）. Results Restraint stress resulted in remarkable mechanical allodynia in the masseter muscle. The head withdrawal threshold was significantly lower in the 7-, 9-, 11- and 14-day stress groups （[0.071 ±0.011], [0.059±0.020], [0.052±0.011], [0.033±0.011] N） than that in the control group （[0.120±0.025] N） （P〈0.05）. Compared to the control group, the rats in the 1-day stress group showed a significant increase of c-fos in neurons of the Vc and then declined to normal level after 1 week gradually. The GFAP expression in astrocytes of the Vc was significantly increased in the 7-, 9-, 11- and 14-day stress groups （4.3±1.0, 4.5±0.6, 4.6±0.5, 4.8±1.3） compared with the control group （2.0±0.8） （P〈0.05）. Conclusions Chronic restraint stress could lower the threshold of mechanical allodynia in the masseter muscle and activate the neurons and astrocytes in Vc. The activation of neurons and astrocytes plays an important role in the masseter hyperalgesia induced by restraint stress in rats.

关 键 词：应激 心理学 咬肌 痛觉过敏 原癌基因蛋白质C-FOS 

分 类 号：R780.2[医药卫生—口腔医学]