转录因子SOX2对胃癌干细胞自我更新、增殖和迁移的影响  

Effect of Transcription Factor SOX2 on the Self-renewal,Proliferation and Migration of Gastric Cancer Stem cell

作　　者：雷超[1] 聂爱英[1] 柯丽[1] 张迪[1] 赵晓迪[1] 时永全[1] 

机构地区：[1]第四军医大学西京消化病医院肿瘤生物学国家重点实验室,陕西西安710032

出　　处：《现代生物医学进展》2017年第7期1220-1224,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81470805);国家自然科学基金青年基金项目(81602641)

摘　　要：目的:检测SOX2在胃癌组织中的表达,探讨SOX2对胃癌干细胞自我更新、增殖和转移能力的影响。方法:采用免疫组化检测SOX2在胃癌及癌旁组织中的表达情况。通过肿瘤球形成实验富集、分离胃癌干细胞。构建SOX2过表达慢病毒并感染胃癌干细胞中,通过实时定量PCR和western bolt检测感染慢病毒后胃癌干细胞中SOX2表达情况。分别利用肿瘤球形成实验检测SOX2对胃癌肿瘤干细胞自我更新能力的影响,CCK-8实验检测SOX2对胃癌干细胞增殖能力的影响,流式细胞术分析SOX2对胃癌干细胞的细胞周期的影响,Transwell实验检测SOX2对胃癌干细胞转移能力的影响。结果:SOX2在胃癌组织中表达显著低于癌旁组织。肿瘤球形成实验能够有效富集胃癌细胞SGC-7901和BGC-823的干细胞。慢病毒载体感染能够显著增强SOX2在胃癌干细胞中的表达。过表达SOX2能够抑制胃癌干细胞的自我更新、增殖和侵袭能力。结论:SOX2在胃癌中发挥抑癌基因的功能,其机制可能通过抑制肿瘤干细胞的自我更新、增殖和侵袭转移能力而抑制胃癌的发生发展。Objective： To investigate the expression of SOX2 in gastric cancer and the role of SOX2 in the self-renewal, prolifera- tion and migration of gastric cancer stem cell. Methods： The expression of SOX2 was detected by immunohistochemical staining in 40 cases of gastric cancer tissues and matched adjacent normal gastric tissues. Tumor sphere formation assay was used to enrich gastric can- cer stem cell. Overexpression of SOX2 in gastric cancer stem cell was mediated by lentivirus infection and confirmed by real-time qPCR and Western blot. Tumor sphere formation assay was used to examine self-renewal ability of gastric cancer stem cell. Cell proliferation was detected with cell counting kit-8 assay. Cell cycle was determined with flow cytometry. Cell migration was assessed with Transwell migration assay. Results： SOX2 was significantly down-regulated in gastric cancer tissues compared with normal gastric tissues. Gastric cancer stem cells were enriched from SGC-7901 and BGC-823 cell lines through tumor sphere formation assays. Real-time qPCR and Western blot analysis indicated forced expression of SOX2 in gastric cancer stem cells by lentivirus infection. Overexpression of SOX2 significantly suppressed the self-renewal, proliferation and migration of gastric cancer stem cells from both SGC-7901 and BGC-823 cells. Conclusion： SOX2 functions as a potential tumor suppressor in gastric cancer and might inhibit gastric carcinogenesis and develop- ment through suppressing self-renewal, proliferation and migration ability of ~astric cancer stem cells.

关 键 词：SOX2 胃癌 增殖 转移 肿瘤干细胞 

分 类 号：R735.2[医药卫生—肿瘤]