噬菌体展示技术与肿瘤靶向治疗  

Phage display technology and tumor targeted therapy

作　　者：侯利丹[1] 孟祥军[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院消化科,200011

出　　处：《肿瘤研究与临床》2017年第3期214-216,共3页Cancer Research and Clinic

基　　金：国家自然科学基金（81272745）;上海市科学技术委员会基金（14140901900）

摘　　要：噬菌体展示技术是通过基因工程手段将外源性基因与噬菌体基因融合,使外源基因编码的多肽或蛋白随噬菌体外壳蛋白的表达展示在噬菌体表面的生物技术.被展示的多肽或蛋白能够保持其相对独立的空间结构和生物活性,与靶分子识别、结合,因此,该技术可作为一种简单有效的筛选工具,成功用于肿瘤靶向肽的筛选、化疗药物的靶向运输、肿瘤抗体的制备等,在肿瘤的靶向治疗研究中发挥重要作用.Phage display technology is a kind of laboratory technique by means of genetic engineering to combine phage with exogenous genes, based on the presentation of a heterogeneous peptide or protein libraries on the surface of phage along with phage coat protein expressions. The displayed proteins or peptides could maintain their own relative spatial structure and biological activity, allowing them to direct phage binding to a target of interest. Therefore, the technology can be widely used as a simple and effective screening tool to screen successfully tumor-targeted peptides, deliver tumor-targeted drugs and prepare tumor antibody, which plays an important role in tumor-targeted therapy.

关 键 词：噬菌体展示 随机多肽库 肿瘤 分子靶向治疗 

分 类 号：R730.5[医药卫生—肿瘤]