1,2,4-三氯苯对金鱼藻氧化压力及生理特性的影响  

Influence of 1,2,4-TCB(trichlorobenzene) on the Oxidative Stress and Physiological Characteristics of Ceratophyllum demersum

作　　者：肖能文[1] 张宇[1,2] 李卫兵[1,2] 高晓奇[1] 盛岩[2] 李俊生[1] 

机构地区：[1]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [2]中国人民大学环境学院,北京100872

出　　处：《环境科学与技术》2017年第1期49-54,共6页Environmental Science & Technology

基　　金：中央级公益性科研院所基本科研业务专项(gyk12611303);国家科技支撑项目(2008BAC43B01)

摘　　要：该实验采用1/10 Hoagland营养液水培法,研究了不同浓度(1.25、2.5、5、10、20和40 mg/L)1,2,4-三氯苯(1,2,4-TCB)对金鱼藻叶绿素含量、可溶性蛋白含量、还原型谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和丙二醛(MDA)含量等的影响。结果表明,不同浓度1,2,4-TCB处理,金鱼藻MDA含量均显著上升且随时间延长MDA上升越显著(p<0.05)。1,2,4-TCB在2.5 mg/L和5 mg/L时GSH含量显著上升。SOD活性在1.25 mg/L和2.5 mg/L时显著上升,金鱼藻受到氧化压力,但达到5 mg/L时,SOD活性显著下降。叶绿素含量在20 mg/L和40 mg/L时显著下降,叶片生理功能受到损伤。研究表明,1,2,4-TCB处理下,金鱼藻生理功能受到抑制,叶绿素含量下降,植株受到氧化胁迫,体内抗氧化酶系统表现防御反应。Effects of different concentrations of 1,2,4-TCB（1,2,4-trichlorobenzene） on the contents of chlorophyll,soluble protein,glutathione（GSH）,malondialdehyde（MDA）,the superoxide dismutase（SOD） and peroxidase（POD） activities in Ceratophyllum demersum were investigated in this study by the method of usage of 1/10 Hoagland nutrient solution hydroponics.The results showed that significantly higher MDA contents in C.demersum were observed in the different concentrations of 1,2,4-TCB（p〈0.05）.MDA content increased significantly along with the time extension（p〈0.05）.GSH content was increased significantly at the concentrations of 2.5 mg/L and 5 mg/L of 1,2,4-TCB compared to control treatment.SOD activity was significantly increased at 1.25 mg/L and 2.5 mg/L relative to control treatment.However,SOD activity was significantly decreased at the concentration of 5 mg/L compared with the control treatment.The Chlorophyll content significantly decreased at 20 mg/L and 40 mg/L,and physiological function for leaf was damaged.Under the treatment with 1,2,4-TCB,C.demersum physiological function was inhibited and chlorophyll content decreased,which indicated that the higher concentration of 1,2,4-TCB,the higher phytotoxicity of 1,2,4-TCB to the C.demersum.

关 键 词：1 2 4-三氯苯 金鱼藻 胁迫 生理特性 

分 类 号：X172[环境科学与工程—环境科学]