MicroRNA快速检测系统的设计  

Design of a Rapid MicroRNA Detection System

作　　者：王燕飞[1,2] 余东升[1] 陈海燕[1] 张战盈[1] 方蔚恺 陆泽橼[1] 李艳蕾[1] 纪玉峰[1] 关一夫[1,3] 徐赤东[1] 江海河[1] 

机构地区：[1]中国科学院合肥物质科学研究院医学物理与技术中心,合肥230031 [2]中国科学技术大学,合肥230026 [3]中国医科大学,沈阳110001

出　　处：《光子学报》2017年第3期179-184,共6页Acta Photonica Sinica

基　　金：国家自然科学基金(No.81371896);安徽省自然科学基金(No.1508085SMC214)资助~~

摘　　要：为了实现MicroRNA的快速检测,设计了一种便携式MicroRNA快速检测仪.基于等温滚环扩增技术,采用光电检测方法,检测标志物受激发出的荧光光强,建立特征荧光分析检测系统.通过改变激发光强度、MicroRNA试剂浓度等参量,验证了该仪器可测量的MicroRNA的浓度范围为0.01~0.1μmol,可检测出的最低检出限为7个拷贝数,MicroRNA浓度与荧光信号强度之间为线性关系(R2=0.999 1).Using Opto-electronic detection technology,marking miRNA and isothermal rolling circle amplification and detecting markers which excite fluorescence intensity,we have designed a new portable device for the rapid detection of miRNA,the recognition system of characteristic fluorescence analysis was established eventually.we have got the point that there is the linear relationship（R2=0.999 1）between miRNA concentration and fluorescence intensity at the time that the miRNA concentration range the instrument can measure is in the range of 0.01~0.1μmol by changing the excitation light intensity,miRNA reagent concentration and other parameters,meanwhile,we also know that it is 7copies that the lowest values the instrument can measure in theory.

关 键 词：生物医学工程 医用光学仪器 核酸检测 荧光 弱光探测 MICRORNA 滚环扩增 

分 类 号：O059[理学] Q6[生物学—生物物理学]