1-甲基-4-苯基-1,2,3,6-四氢吡啶诱导小鼠帕金森病模型中Th-17细胞因子的研究（英文）  

1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Th-17 cytokines in mice

作　　者：赵璇君[1] 林金飞[1] 徐一达[2] 林凌云[3] 赵盈盈[3] 申延琴[2] 

机构地区：[1]汕头大学医学院神经科学中心,广东汕头515041 [2]江南大学无锡医学院,江苏无锡214122 [3]汕头大学精神卫生中心,广东汕头515065

出　　处：《延安大学学报（医学科学版）》2017年第1期4-7,共4页Journal of Yan'an University:Medical Science Edition

基　　金：supported by NNSF of China(81072622)~~

摘　　要：目的为了探究1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)是否可引起小鼠体内Th17细胞因子IL-17和IL-23的表达变化。方法对8周龄C57BL/6J小鼠腹腔注射MPTP或PBS,于第0,1,2,5,7天进行前爪伸屈试验。并第8天处死后收集脑组织内纹状体,并检测血清中炎症因子的含量。结果实验结果表明,较PBS处理组,MPTP处理组雌、雄鼠的伸爪频率下降,并在处理后第1天有显著性差异,且在第2~7天的伸爪频率并没有改善,提示MPTP对于伸爪能力有抑制作用。在雄性小鼠中,经过MPTP处理后,IL-17在纹状体内的含量达到14900±330 ng/mg蛋白(对照组为12420±914 ng/mg蛋白),在血清的含量达到1970±145 ng/ml(对照组为1400±132 ng/ml)。然而雌性小鼠中未见IL-17的差异。IL-23在MPTP处理后的小鼠纹状体和血清中未见差异。结论本实验发现MPTP处理后的小鼠纹状体和血清中IL-17的表达上升,而IL-23未见明显变化,提示IL-17可能在小鼠PD模型中具有一定的作用。Objective To investigate whether 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP） induces IL-17 and IL-23 in a murine model.Methods 8-week-old C57BL/6J mice were intraperitoneally injected with MPTP or PBS,subjected to a paw reaching test on days 0,1,2,5 and 7.On day 8,the mice were euthanized and,striata were dissected and serum were collected for cytokines assay.Results The results showed that MPTP treatment decreased the rate of both male and female mice in the paw reaching test.Significance was found at day 1 after MPTP treatment.PBS-treated mice demonstrated improvement by their rate between d2 ~ d7,whereas MPTP-treated mice did not.IL-17 levels were increased both in striatum（14900 ± 330 ng/mg protein VS.12420 ± 914 ng/mg protein） and serum（1970 ± 145 ng/ml VS.1400 ± 132 ng/ml） of MPTP-treated male mice,but not female mice,however,IL-23 levels either in the striatum or in serum were unchanged by MPTP treatment.Conclusion This work argued that MPTP treatment induces IL-17 but not IL-23 in mice,both in brain and peripheral blood.

关 键 词：帕金森病 MPTP 小鼠 TH17 性别 

分 类 号：R742.5[医药卫生—神经病学与精神病学]