Genetic biosensors for small-molecule products： Design and applications in high-throughput screening  

作　　者：Qingzhuo Wang Shuang-Yan Tang Sheng Yang 

机构地区：[1]Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai 200025, China [2]University of the Chinese Academy of Sciences, Beijing 100049, China [3]CAS Key Laboratory of Microbial Physiological and Metebolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [4]Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China

出　　处：《Frontiers of Chemical Science and Engineering》2017年第1期15-26,共12页化学科学与工程前沿（英文版）

摘　　要：Overproduction of small-molecule chemicals using engineered microbial cells has greatly reduced the production cost and promoted environmental protection. Notably, the rapid and sensitive evaluation of the in vivo concentrations of the desired products greatly facilitates the optimization process of cell factories. For this purpose, many genetic components have been adapted into in vivo biosensors of small molecules, which couple the intracel- lular concentrations of small molecules to easily detectable readouts such as fluorescence, absorbance, and cell growth. Such biosensors allow a high-throughput screen- ing of the small-molecule products, and can be roughly classified as protein-based and RNA-based biosensors. This review summarizes the recent developments in the design and applications of biosensors for small-molecule products.Overproduction of small-molecule chemicals using engineered microbial cells has greatly reduced the production cost and promoted environmental protection. Notably, the rapid and sensitive evaluation of the in vivo concentrations of the desired products greatly facilitates the optimization process of cell factories. For this purpose, many genetic components have been adapted into in vivo biosensors of small molecules, which couple the intracel- lular concentrations of small molecules to easily detectable readouts such as fluorescence, absorbance, and cell growth. Such biosensors allow a high-throughput screen- ing of the small-molecule products, and can be roughly classified as protein-based and RNA-based biosensors. This review summarizes the recent developments in the design and applications of biosensors for small-molecule products.

关 键 词：BIOSENSOR small molecule product transcrip-tion factor RIBOSWITCH high-throughput screening 

分 类 号：Q78[生物学—分子生物学] TP212.3[自动化与计算机技术—检测技术与自动化装置]