Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH： A mini review  

作　　者：Jongmoon Park Yunnam Choi 

机构地区：[1]Department of Chemical Engineering, Pohang University of Science and Technology, Gyeongbuk 790-784, Korea [2]School of Environmental Science and Engineering, Pohang University of Science and Technology, Gyeongbuk 790-784, Korea [3]Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, Gyeongbuk 790-784, Korea

出　　处：《Frontiers of Chemical Science and Engineering》2017年第1期66-71,共6页化学科学与工程前沿（英文版）

摘　　要：Cyanobacteria can produce useful renewable fuels and high-value chemicals using sunlight and atmo- spheric carbon dioxide by photosynthesis. Genetic manip- ulation has increased the variety of chemicals that cyanobacteria can produce. However, their uniquely abundant NADPH-pool, in other words insufficient supply of NADH, tends to limit their production yields in case of utilizing NADH-dependent enzyme, which is quite common in heterotrophic microbes. To overcome this cofactor imbalance and enhance cyanobacterial fuel and chemical production, various approaches for cofactor engineering have been employed. In this review, we focus on three approaches： （1） utilization of NADPH- dependent enzymes, （2） increasing NADH production, and （3） changing cofactor specificity of NADH-dependent enzymes from NADH to NADPH.Cyanobacteria can produce useful renewable fuels and high-value chemicals using sunlight and atmo- spheric carbon dioxide by photosynthesis. Genetic manip- ulation has increased the variety of chemicals that cyanobacteria can produce. However, their uniquely abundant NADPH-pool, in other words insufficient supply of NADH, tends to limit their production yields in case of utilizing NADH-dependent enzyme, which is quite common in heterotrophic microbes. To overcome this cofactor imbalance and enhance cyanobacterial fuel and chemical production, various approaches for cofactor engineering have been employed. In this review, we focus on three approaches： （1） utilization of NADPH- dependent enzymes, （2） increasing NADH production, and （3） changing cofactor specificity of NADH-dependent enzymes from NADH to NADPH.

关 键 词：NADH-dependent enzyme NADPH-depen-dent enzyme TRANSHYDROGENASE site-directed mutagenesis enzyme engineering 

分 类 号：TQ922.1[轻工技术与工程—发酵工程] Q552[生物学—生物化学]