Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis in Gluconobacter oxydans WSH-003  

作　　者：Hui Wan Yu Xia Jianghua Li Zhen Kang Jingwen Zhou 

机构地区：[1]School of Biotechnology and Key Laboratory of Industrial B iotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China [2]Synergetic Innovation of Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China

出　　处：《Frontiers of Chemical Science and Engineering》2017年第1期72-88,共17页化学科学与工程前沿（英文版）

基　　金：This work was supported by grants from the National High Technology Research and Development Program of China （863 Program, 2012AA022103）, the National Basic Research Program of China （973 Program, 2013CB733602, 2014CB745100）, the Major Program of National Natural Science Foundation of China （Grant No. 21390204）, the Program for New Century Excellent Talents in University （NCET-12-0876）, the Foundation for the Author of National Excellent Doctoral Dissertation of China （FANEDD, 201256）, the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the 111 Project （111-2-06）.

摘　　要：Pyrroloquinoline quinone （PQQ） plays a sig- nificant role as a redox cofactor in combination with dehydrogenases in bacteria. These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry, such as vitamin C production. While biosynthesis of PQQ genes has been widely studied, PQQ-transport mechanisms used both two-dimensional remain unclear. Herein, we fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects ofpqqB overexpres- sion in an industrial strain of Gluconobacter oxydans WSH-003. We have identified 73 differentially expressed proteins and 99 differentially expressed genes, a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis. We also described several putative candidate effectors that responded to increased PQQ levels resulting from pqqB overexpression. Furthermore, quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains, and the results showed that ompW, B932 1930 and B932_2186 were upregulated in all conditions. Then the three genes were over-expressed in G. oxydans WSH-003 and PQQ production were detected. The results showed that extracellular PQQ of B932_1930 （a transporter） and B932_2186 （an ABC transporter perrnease） overexpression strains were enhanced by 1.77- fold and 1.67-fold, respectively. The results suggest that the proteins encoded by PqqB, B932_1930 and B932 2186 might enhance the PQQ secretion process.Pyrroloquinoline quinone （PQQ） plays a sig- nificant role as a redox cofactor in combination with dehydrogenases in bacteria. These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry, such as vitamin C production. While biosynthesis of PQQ genes has been widely studied, PQQ-transport mechanisms used both two-dimensional remain unclear. Herein, we fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects ofpqqB overexpres- sion in an industrial strain of Gluconobacter oxydans WSH-003. We have identified 73 differentially expressed proteins and 99 differentially expressed genes, a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis. We also described several putative candidate effectors that responded to increased PQQ levels resulting from pqqB overexpression. Furthermore, quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains, and the results showed that ompW, B932 1930 and B932_2186 were upregulated in all conditions. Then the three genes were over-expressed in G. oxydans WSH-003 and PQQ production were detected. The results showed that extracellular PQQ of B932_1930 （a transporter） and B932_2186 （an ABC transporter perrnease） overexpression strains were enhanced by 1.77- fold and 1.67-fold, respectively. The results suggest that the proteins encoded by PqqB, B932_1930 and B932 2186 might enhance the PQQ secretion process.

关 键 词：2D-DIGE pqqB pyrroloquinoline quinone RNA-Seq Vitamin C 

分 类 号：Q51[生物学—生物化学] TQ924[轻工技术与工程—发酵工程]