过表达血红素加氧酶-1、金属硫蛋白2A、泛素蛋白B、核糖体蛋白S27a对HepG2细胞增殖和侵袭力的影响  

Overexpression of HMOX1,MT2A,UBB,RPS27a on the proliferation and invasion of HEPG2 cells

作　　者：吴学威[1] 钟裕恒 黄湘[1] 谭家余[2] 李冬秀[1] 

机构地区：[1]南方医科大学附属中山博爱医院产前诊断中心,广东中山528403 [2]南方医科大学附属中山博爱医院中心ICU,广东中山528403

出　　处：《中国卫生检验杂志》2017年第6期761-766,共6页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金(81101534);广东省自然科学基金(S2012010010824)

摘　　要：目的研究过表达目标蛋白(HMOX-1、MT2A、UBB、RPS27a)对人肝癌HepG2细胞增殖和侵袭力的影响。方法用目标蛋白的过表达质粒转染HepG2细胞株,用空载体转染HepG2作为对照组。应用MTT法测定细胞体外增殖能力;Transwell实验检测各组细胞的体外侵袭力。结果 MTT法结果提示过表达目标蛋白后,HepG2细胞株增殖能力较对照组明显增强,差异有统计学意义(P<0.05);Transwell实验结果表明,过表达HMOX1、RPS27a后,HepG2细胞侵袭能力较对照组明显增加,差异有统计学意义(P<0.05);过表达MT2A、UBB后,HepG2细胞侵袭能力与对照组比较差异无统计学意义(P>0.05)。结论过表达目标蛋白可增强HepG2细胞的增殖和侵袭能力。Objective To study the effect of target proteins（ MT2 A,HMOX-1,RPS27 a,UBB） on the proliferation and invasion of human hepatocellular carcinoma HepG2 cells. Methods The target protein over-expression plasmids were transfected with the HepG2 cell lines,and HepG2 transfected with empty vector was admitted as the negative control group. MTT assay was used to detect the proliferation of cells in vitro,and the invasion ability of the cells was detected by Transwell assay.Results MTT assay results suggested that after over-expression of the target protein,the proliferation ability of HepG2 cells was significantly enhanced compared with that of control group,and the difference was statistically significant（ P〈0. 05）; Transwell experiment results showed that after overexpression of HMOX1,RPS27 a,the invasiveness of HEPG2 cell increased significantly compared with the control group,and the difference was statistically significant（ P〈0. 05）; after overexpression of MT2 A,UBB,the invasiveness of HEPG2 cell did not increased obviously compared with the control group,with the difference not statistically significant（ P〉0. 05）. Conclusion Overexpression of target protein can enhance the proliferation and invasion of HepG2 cells.

关 键 词：HEPG2细胞 血红素加氧酶-1 金属硫蛋白2A 泛素蛋白B 核糖体蛋白S27a 

分 类 号：R735.7[医药卫生—肿瘤]