CBX8过表达或沉默对人神经胶质瘤细胞增殖和凋亡的影响  

Effects of silencing and overexpression of CBX8 on proliferation and apoptosis of human glioma cells

作　　者：张百平[1] 孙树凯[1] 贾栋[1] 

机构地区：[1]第四军医大学唐都医院神经外科,陕西西安710038

出　　处：《中国病理生理杂志》2017年第4期723-729,共7页Chinese Journal of Pathophysiology

摘　　要：目的:探究染色质结构域蛋白8(chromodomain protein 8,CBX8)对人神经胶质瘤细胞增殖与凋亡的作用。方法:Western blot和RT-qPCR检测组织及细胞系中CBX8的表达。构建过表达CBX8和沉默CBX8载体,转染神经胶质瘤细胞T98G和U87MG,分别用MTT法和BrdU实验检测细胞增殖,流式细胞术检测细胞凋亡。结果:与正常脑组织和星形胶质细胞相比,神经胶质瘤组织及细胞中的CBX8蛋白和mRNA水平明显上升。在T98G和U87MG细胞中,过表达CBX8均促进细胞增殖,抑制细胞凋亡,并上调Rb/E2F1的表达水平,而沉默CBX8则作用相反。sh-E2F1转染细胞之后,cyclin D1的表达以及Bcl-2/Bax的比值降低。结论:CBX8可能通过Rb/E2F1通路调节胶质瘤细胞的增殖和凋亡。AIM： To explore the effects of chromodomain protein 8（ CBX8） on the proliferation and apoptosis of human glioma cells. METHODS： The expression of CBX8 in the tissues and cells was detected by Western blot and RT-qPCR. The overexpression（ Flag-CBX8） and silencing（ sh-CBX8） vectors of CBX8 were constructed and transfected into glioma T98 G cells and U87 MG cells. The cell proliferation was detected by MTT assay and BrdU staining. The cell apoptosis was analyzed by flow cytometry. The protein expression of Rb/E2F1 was detected by Western blot. RESULTS：Compared with normal brain tissues and astrocytes,the expression of CBX8 was increased in the glioma tissues and glioma cells. Overexpression of CBX8 promoted the cell proliferation,inhibited the cell apoptosis,and upregulated the protein levels of Rb/E2F1. On the contrary,silencing of CBX8 inhibited the cell proliferation,promoted the cell apoptosis,and decreased the protein levels of Rb/E2F1 in the T98 G cells and U87 MG cells. Moreover,the expression of cyclin D1 and Bcl-2/Bax ratio were reduced after transfection with sh-E2F1 in the T98 G cells and U87 MG cells. CONCLUSION： CBX8 may regulate the proliferation and apoptosis of glioma cells through Rb/E2F1 pathway.

关 键 词：神经胶质瘤 色素框同源物8 T98G细胞 U87MG细胞 

分 类 号：R730.23[医药卫生—肿瘤] R739.4[医药卫生—临床医学]