基于层次分析法评分的多指标正交试验法优选宣木瓜的醇水双提工艺  

Optimization of double extraction techniques by alcohol and water for Chaenomelis fructus from Xuancheng using Orthogonal design method

作　　者：张玲[1] 查日维[2] 谢晓梅[1] 陈浩[3] 姚勇 

机构地区：[1]安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥230012 [2]无锡市第二人民医院,江苏无锡214002 [3]安徽中医药大学第一附属医院,安徽合肥230031 [4]宣城市金泉生态农业有限责任公司,安徽宣城242000

出　　处：《华西药学杂志》2017年第2期189-192,共4页West China Journal of Pharmaceutical Sciences

基　　金：十二五国家科技支撑计划(2011BAI04B06);安徽省高校自然科学研究重点项目(KJ2015A128);安徽中医药大学校级探索性科研项目(2016ts074)

摘　　要：目的筛选宣木瓜的最佳提取工艺。方法采用正交试验法,以齐墩果酸和熊果酸、总有机酸、总多酚和总多糖提取量为指标;层次分析法确定权重,多指标综合评分优选宣木瓜醇水双提的工艺参数。结果最佳提取工艺为:8倍量75%乙醇回流提取2次,每次2 h,药渣再用12倍量水回流提取2 h。结论优选工艺为宣木瓜提取物及配方颗粒制备奠定了基础。OBJECTIVE To establish an optimized extraction technique for Chaenomelis fructus from Xuancheng. METHODS The extraction process was tested by alcohol and water using Orthogonal design. The contents of oleanolic acid ＆ ursolie acid, total organic acid, total polyphenols and total polysaccharide in C. fructus were determinated and used as the evaluation parameter of the extracts. Analytical Hierarchy Process （AHP） was used to determine the weight coefficient. RESULTS The optimum conditions of extraction were therefore determined as follows：Reflux extraction method was used ,8 -fold ethanol with 75% concentration was used to extract for 2 times and each time 2 hours. Then the residue was extracted with 12 - fold water for 2 hours. CONCLUSION The optimized extrac- tion procedure can ensure the preparation of the extracts and formula granule of C. fructus.

关 键 词：宣木瓜 醇水双提 正交设计 层次分析法 齐墩果酸 熊果酸 总有机酸 总多酚 总多糖 

分 类 号：R917[医药卫生—药物分析学]