微小RNA-223对人胰腺癌PANC-1和miaPaCa-2细胞增殖和迁移的影响  

Effect of microRNA－223 on proliferation and migration of pancreatic cancer cell lines PANC－1 and miaPaCa－2

作　　者：黄成[1,2] 熊龙辉[1] 许毓敏[1] 胡钶[1] 马广念[1] 陈新斌 刘大威 贺德[1] 

机构地区：[1]南方医科大学附属深圳宝安医院普通外科,深圳518101 [2]广东医科大学研究生学院,湛江524023

出　　处：《中华实验外科杂志》2017年第3期372-374,共3页Chinese Journal of Experimental Surgery

基　　金：深圳市科技创新委员会基础研究项目（JCYJ20140414103937769）

摘　　要：目的 探讨微小RNA（miRNA,miR）-223对胰腺癌细胞增殖和迁移的调控.方法 将miR-223模拟物和抑制物转染胰腺癌PANC-1和miaPaCa-2细胞株,通过实时荧光定量聚合酶链反应（FQ-PCR）检测miR-223的表达情况;噻唑蓝（MTT）法检测miR-223对细胞增殖的作用;Transwell实验检测细胞的迁移能力.结果 miR-223在胰腺癌PANC-1和miaPaCa-2细胞中相对表达水平明显高于正常胰腺细胞（8.21±0.83、11.22±0.72,tPANC-1=14.886,P=0.000;tmiaPaCa-2=24.025,P=0.000）;转染miR-223模拟物后,胰腺癌PANC-1和miaPaCa-2细胞内miR-223相对表达量明显上调（8.18±0.82、10.51±0.79,tPANC-1=15.221,P=0.001;tmiaPaCa-2=20.765,P=0.001）;MTT和Transell实验结果显示,过表达miR-223能明显促进PANC-1和miaPaCa-2细胞的增殖（1.43±0.07比0.49±0.08,t=15.715,P=0.000;1.55±0.07比0.63±0.05,t=19.283,P=0.000）和迁移[（164.20±8.45）个比（73.50±4.50）个,t=16.419,P=0.000;（133.45±7.75）个比（56.40±6.25）个,t=13.401,P=0.000];敲低miR-223表达后,胰腺癌PANC-1和miaPaCa-2细胞的增殖比例明显降低（0.73±0.06比1.04±0.05,t=6.754,P=0.000;0.77±0.04比1.13±0.08,t=7.321,P=0.000）,同时迁移速度明显减慢[（35.30±3.36）个比（72.60±4.10）个,t=16.419,P=0.000;（32.50±2.75）个比（57.40±3.35）个,t=9.953,P=0.000].结论 miR-223具有促进胰腺癌PANC-1和miaPaCa-2细胞株增殖和迁移的作用.Objective To identify the effects of microRNA （miRNA, miR）-223 on proliferation and migration of pancreatic cancer cells.Methods miR-223 mimic and inhibitor were transfected into PANC-1 and miaPaCa-2 cell lines.The expression level of miR-223 was determined by real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）.Cell proliferation was evaluated by methyl thiazol tetrazolium （MTT） assay.Transwell migration assy was performed for cell migration ability.ResultsThe expression of miR-223 in the PANC-1 and miaPaCa-2 cell lines was significantly higher than that in normal pancreatic cells （8.21±0.83, 11.22±0.72, tPANC-1=14.886, P=0.000;tmiaPaCa-2=24.025, P=0.000）.FQ-PCR showed the miR-223 expression level in PANC-1 and miaPaCa-2 cell lines were increased significantly after transfection with miR-223 mimics （8.18±0.82, 10.51±0.79, tPANC-1=15.221, P=0.001;tmiaPaCa-2=20.765, P=0.001）.The number of pancreatic cancer cells in miR-223 tranfected group was extremely increased as compared with control group （1.43±0.07 vs.0.49±0.08, t=15.715, P=0.000;1.55±0.07 vs.0.63±0.05, t=19.283, P=0.000）.The number of migrated PANC-1 and miaPaCa-2 cells in miR-223 tranfected group was significantly greater than that in miR-23 control group （164.20±8.45 vs.73.50±4.50, t=16.419, P=0.000;133.45±7.75 vs.56.40±6.25, t=13.401, P=0.000）.The proliferation of pancreatic cancer was significantly decreased in miR-223 inhibitor transfected group as compared with that in miR-23 control group （0.73±0.06 vs.1.04±0.05, t=6.754, P=0.000;0.77±0.04 vs.1.13±0.08, t=7.321, P=0.000）.Transell results showed less cells in miR-223 inhibitor transfected group than in control group （35.30±3.36 vs.72.60±4.10, t=16.419, P=0.000;32.50±2.75 vs.57.40±3.35, t=9.953, P=0.000）.Conclusion The overexpression of miR-223 could remarkably promote the proliferation and migration abilities of human pancreatic cancer PANC-1 and miaPaCa-2 cells.

关 键 词：微小RNA-223 胰腺癌 增殖 迁移 

分 类 号：R735.9[医药卫生—肿瘤]