自噬对人胶质细胞瘤细胞上皮-间充质转化和侵袭力的影响  

Influence of autophagy on epithelial-mesenchymal transition and invasion of glioma cells

作　　者：熊俊[1] 张媚[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022 [2]武汉市中心医院神经内科,430022

出　　处：《中华实验外科杂志》2017年第3期417-419,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金（2014CFC1109）

摘　　要：目的 观察自噬对人胶质细胞瘤上皮-间充质转化（EMT）的作用和对其侵袭力的影响.方法 以Hank＇s平衡盐缓冲液营养剥夺培养人胶质细胞瘤细胞株SHG-44,以诱导自噬,观察SHG-44细胞上皮间质标志表达和侵袭力变化.同时构建转染针对自噬基因Atg3的小干扰RNA（siRNA-Atg3）,抑制营养剥夺条件下SHG-44细胞自噬,分析自噬抑制前后,SHG-44细胞上皮间质标志表达和侵袭力变化,探讨自噬对人胶质细胞瘤细胞EMT和侵袭力的影响.结果 平衡盐缓冲液显著诱导人胶质细胞瘤SHG-44细胞自噬,其上皮标志胶质纤维酸性蛋白（GFAP）表达下调,而间质标志波形蛋白（Vimentin）和基质金属蛋白酶-2（MMP-2）表达上调,细胞侵袭数显著增加[（18100±1500）个比（68750±3350）个,t=864.345,P=0.001].对SHG-44细胞转染siRNA-Atg3后,营养剥夺诱导的自噬活性明显受抑制,较对照组细胞,未出现明显上皮间质标志表达改变,细胞侵袭数也显著下降[（68750±3350）个比（25450±1450）个,t=565.441,P=0.001].结论 自噬可显著诱导人胶质细胞瘤细胞EMT和促进其侵袭.Objective To investigate the influence of autophagy on epithelial-mesenchymal transition （EMT） and invasion of glioma cells.Methods Autophagy of glioma cell line, SHG-44, was induced by starvation in Hank＇s balanced salt solution （HBSS）.The change in markers expression and invasion of SHG-44 cells was observed.The small interfering RNA （siRNA） of Atg3 was constructed and transfected into SHG-44 cells to inhibit autophagy under starvation.The change in the markers expression and invasion after transfection was compared to investigate the role of autophagy in EMT and invasion of SHG-44 cells.Results HBSS significantly induced autophagy of SHG-44 cells.The expression of epithelial marker, glia fibrillary acidic protein （GFAP） was downregulated whereas the expression of mesenchymal markers, Vimentin and matrix metalloproteinase （MMP）-2 was notably upregulated.The invasion number of cells was also significantly increased （18 100±1 500 vs.68 750±3 350, t=864.345, P=0.001）.After transfection of siRNA-Atg3, the starvation-induced autophagy of SHG-44 cells was obviously inhibited.In contrast to the control cells, there was no notable change in epithelial and mesenchymal markers expression, and the invasion number of SHG-44 cells was also significantly reduced （68 750±3 350 vs.25 450±1 450, t=565.441, P=0.001）.Conclusion Autophagy significantly induces EMT of glioma cells and promote their invasion.

关 键 词：胶质细胞瘤 自噬 上皮-间充质转化 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]