电喷雾质谱法对几种氨基酸的快速检测  

The rapid analysis of amino acids by electrospray ionization mass spectrometry

作　　者：聂龙辉[1] 王卫国[2] 

机构地区：[1]湖北工业大学材料与化学工程学院,武汉460068 [2]中国科学院大连化学物理研究所,大连116023

出　　处：《分析试验室》2017年第4期404-406,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(51572074)资助

摘　　要：利用电喷雾质谱技术对甘氨酸、N-(羧甲基)甘氨酸、谷氨酸混合样品进行了检测,考察了电离源的喷雾电压、喷雾气压、进样流速对检测结果的影响规律。结果表明,以1:1(V/V)的甲醇水溶液作为溶剂时,甘氨酸、N-(羧甲基)甘氨酸、谷氨酸能够有效地被电离,产物离子均为母体离子结合一个质子(M^+H)^+。当电喷雾电压从1 kV增加到6 kV过程中,离子信号强度迅速增加,增大到5 kV后基本保持不变。注入流速在1~5μL/min间信号强度随注入流速增加而迅速增大,之后基本保持不变。喷雾气压低于4 MPa时,信号不稳定;在4~12 MPa气压范围内信号稳定且对信号强度影响不大。The measurement of amino acids is important for the diagnosis of the diseases. Using electrospray ionization mass spectrometry, the mixtures of Glycine, N-（ carboxymethyl）-Glycine, and glutamic acid were detected. The effects of operational parameters on the signal intensity were investigated in details. The results indicated that Glycine, N-（carbexymethyl）-Glycine, and glutaznic acid could be ionized efficiently in the solvent of water-methanol （1：1, V/V）. And the main product ions were （M ＋ H） ^＋. With the increase of electrical voltage from 1 kV to 6 kV, the signal increased monotonously, and then remained unchanged at 5 kV. Similar tendency was observed with rising flow rate： from i -5 μL/min. No striking differences were observed when the nitrogen pressure varied in the range of 4 -12 MPa.

关 键 词：电喷雾质谱 氨基酸 电离源 检测 

分 类 号：R446[医药卫生—诊断学] O657.63[医药卫生—临床医学]