干扰素诱导蛋白AIM2在乳腺癌细胞系及乳腺癌组织中的表达及意义  

The expression and significance of absent in melanoma AIM2 in breast cancer cell lines and breast cancer tissues

作　　者：刘志勇[1] 易坚[1] 刘凤恩[1] 

机构地区：[1]赣南医学院第一附属医院,江西赣州341000

出　　处：《赣南医学院学报》2017年第1期46-49,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：2015年江西省教育厅科学技术研究项目(编号:150942)

摘　　要：目的:研究干扰素诱导蛋白AIM2(absent in melanoma 2)在乳腺癌细胞系及乳腺癌组织中的表达及意义。方法:建立MCF-7 t TA-AIM2细胞为实验组,MCF-7 t TA-Luc细胞为对照组。以RT-PCR分析在不同的乳腺癌细胞株以及乳腺癌组织中AIM2基因的表达。利用TUNEL检测细胞凋亡。结果:本实验建立的Tet-off诱导系统可使t TA结合到TRE,从而促进AIM2基因转录,进而使AIM2蛋白能大量表达。AIM2基因表达量在MCF-10A、184A1细胞株中非常低,在MDA-MB-435中最高。同时,乳腺癌组织较邻近正常乳房组织有较多AIM2基因表达。AIM2可促进乳腺癌细胞凋亡。结论:在乳腺癌Tet-off诱导系统中,AIM2可促进乳腺癌细胞凋亡,同时在MDA-MB-435及乳腺癌组织中的表达较高。Objective： To study the expression and significance of absent in melanoma AIM2 in breast cancer cell line and breast cancer tissue. Methods： MCF-7 tTA-AIM2 cells and MCF-7 tTA-Luc were established as the experimental group and control group respectively. RT-PCR was used to analyze the AIM2 gene expression in different breast cancer cell lines and in breast cancer tissue. Apoptosis was detected by staining with TUNEL. Results： This experiment established a Tet- off guidance system which can make the tTA to TRE, promote AIM2 gene transcription, and upregulate the AIM2 protein multiplicity expression. AIM2 gene expression data in MCF-10A, 184A1 cell lines was very low, and the highest in the MDA-MB-435. At the same time, the breast tissues have more AIM2 gene expression than the adjacent normal breast tis- sues. AIM2 expression can significantly promote the apoptosis of breast cancer ceils. Conclusion： In breast cancer in- duced by Tet-off system, AIM2 can promote breast cancer cells apoptosis, at the same time in MDA-MB-435 and in breast cancer tissue has high expression.

关 键 词：干扰素诱导蛋白 乳腺癌细胞 乳腺癌组织 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]