米邦塔仙人掌多糖提取工艺的研究  

Studies of Extraction Technique of Polysaccharide from Opuntia milpa alta Haw

作　　者：徐丛玥[1] 林款 茹琴[1] 梁征[1] 李超英[1,2] 

机构地区：[1]江汉大学武汉生物医学研究院,武汉430056 [2]汉济生物科技(武汉)有限公司,武汉430075

出　　处：《湖北农业科学》2017年第5期927-931,共5页Hubei Agricultural Sciences

基　　金：欧阳康乐产学研用创新基金项目

摘　　要：对热水提取米邦塔仙人掌(Opuntia milpa alta Haw)粗多糖的提取工艺进行研究。以新鲜米邦塔仙人掌为原料,采用单因素试验考察料液比、提取温度、提取时间和提取次数对米邦塔仙人掌粗多糖得率的影响,并通过响应面法优化工艺参数。结果表明,影响粗多糖得率的因素主次顺序为提取温度>料液比>提取时间。确定最佳提取工艺参数为提取温度83℃,料液比1∶28(g∶m L),提取时间为3 h,在此条件下,仙人掌粗多糖的得率为2.05%。Polysaccharide in fresh Opuntia milpa alta Haw was extracted with hot water. The effects of the ratio of material to water,extraction temperature,extraction time and extraction times on the yield of polysaccharide were investigated by single factor experiments. Subsequently,a quadratic regression rotational combinational design was employed,and data obtained were analyzed with response surface methodology. Polysaccharide yield was most significantly affected by extraction temperature,followed by water to material ratio,and extraction time. An optimum polysaccharide yield of 2.05% was obtained when opuntia milpa alta was treated at 83 ℃ for 3 h in a material-to-water ratio of 1∶28（g∶m L）.

关 键 词：米邦塔仙人掌(Opuntia milpa alta Haw) 粗多糖 提取 响应面法 

分 类 号：S682.33[农业科学—观赏园艺] TS201.1[农业科学—园艺学]