电针抗下肢肌萎缩的频率组合优化及其机制的初步研究  

A Preliminary Study of the Optimal Frequency Combination and Mechanism of Electroacupuncture Treatment for Lower Limb Myatrophy

作　　者：庄伊洢[1] 黄晓卿[1] 叶笑然[1] 陈玄[1] Zhuang Yi-yi HUANGXiao-qing YE Xiao-ran CHEN Xuan(Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003,Chin)

机构地区：[1]福建省中医药研究院,福州350003

出　　处：《上海针灸杂志》2017年第4期473-477,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金面上项目(81373733);福建省属公益类科研院所基本科研专项(2015R1035-4)

摘　　要：目的筛选电针治疗肌萎缩的优化电脉冲参数并探讨机理。方法 SD大鼠40只,制作下肢肌萎缩模型后随机分为A组(模型组,不电针)、B组(波宽0.5 ms,频率2 Hz)、C组(波宽200 ms,频率2 Hz)、D组(波宽200 ms,频率1/2/3 Hz)。电针足三里和承山穴,共4个疗程。观察坐骨神经功能指数(SFI)、腓肠肌湿重比(GW)、腓肠肌纤维横截面积比(GC)、腓肠肌细胞直径比(GD)和腓肠肌细胞凋亡指数(AI)的变化。结果与A组比,C组和D组的SFI、GW、GC明显增大,AI明显减小(P<0.05);与B组比,C组、D组的AI明显减小(P<0.05);与C组比,D组的AI明显减小(P<0.05)。结论以波宽200 ms,频率1/2/3 Hz的电脉冲延缓肌萎缩为优化电针参数,其机制与抑制肌细胞凋亡有关。Objective To sift out the optimized electric pulse parameters for electroacupuncture treatment for lower limb myatrophy and explore the mechanism of its treatment.Method A model of lower limb myatrophy was made in 40 SD rats.The rats were then randomized to groups A （model group without electroacupuncture）, B （a pulse duration of 0.5 ms and a frequency of 2 Hz）, C （a pulse duration of 200 msand a frequency of 2 Hz） and D （a pulse duration of 200 ms and a frequency of 1/2/3 Hz）.Points Zusanli（ST36）and Chengshan（BL57）were given electroacupuncture for four courses of treatment.Thesciaticfunction index （SFI）,gastrocnemius muscle wet weight ratio （GW）, gastrocnemius muscle fiber cross-sectional area ratio （GC）,gastrocnemius muscle cell diameter ratio （GD）and gastrocnemius muscle cell apoptosis index （AI）were determined.Result SFI, GW and GC increased significantly and AI decreased significantly in groups C and D compared with group A （P〈0.05）. AI decreased significantly in groups C and D compared with group B （P〈0.05）. AI decreased significantly in groups D compared with group C （P〈0.05）. Conclusion Apulse duration of 200 ms and a frequency of 1/2/3 Hz are the optimizedelectric pulse parametersfor electroacupuncture delaying myatrophy.Its mechanism is related to inhibiting myocyte apoptosis.

关 键 词：针刺 电针 波宽 频率 肌萎缩 细胞凋亡 大鼠 

分 类 号：R2-03[医药卫生—中医学]