紧密连接蛋白Occludin及ZO-1在根除幽门螺杆菌慢性胃炎组织中的表达  

Expressions of tight junction protein Occludin and ZO-1 in patients with chronic gastritis of Helicobacter pylori infection

作　　者：樊叶[1] 汪志兵[1] 关月 韩维维[1] 姜宗丹[1] 王劲松[3] 黄文斌[3] 张振玉[1] 

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)消化科,江苏南京210006 [2]解放军南京总医院消化内科 [3]南京医科大学附属南京医院(南京市第一医院)病理科,江苏南京210006

出　　处：《胃肠病学和肝病学杂志》2017年第4期440-443,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的观察闭锁蛋白(Occludin)及闭锁小带蛋白(zonula occludens-1,ZO-1)在根除幽门螺杆菌(Helicobacter pylori,H.pylori)慢性胃炎组织中的表达变化,探讨其与患者临床病理及预后的关系。方法采用免疫组织化学法检测H.pylori阴性正常人(25例)、根除H.pylori前后慢性浅表性胃炎(30例)及慢性萎缩性胃炎(30例)患者胃窦黏膜标本中Occludin、ZO-1的蛋白表达。结果 H.pylori阴性的正常人胃黏膜Occludin、ZO-1蛋白表达较H.pylori阳性慢性胃炎患者高,差异有统计学意义(P<0.05),H.pylori阳性的慢性浅表性胃炎组织Occludin及ZO-1蛋白表达较慢性萎缩性胃炎增强,差异有统计学意义(P<0.05);H.pylori根除治疗后,慢性浅表性胃炎组织中Occludin及ZO-1表达量较治疗前增强(P<0.05),慢性萎缩性胃炎组织Occludin、ZO-1表达无明显变化(P>0.05);H.pylori未根除患者,Occludin及ZO-1蛋白变化差异无显著性(P>0.05)。结论 H.pylori阳性的慢性胃炎患者胃黏膜屏障受损,萎缩发生前根除H.pylori治疗可提高Occludin及ZO-1蛋白表达,进而修复H.pylori引起的胃黏膜损伤。Objective To observe the effect of Helicobacter pylori （H. priori） infection on expressions of tight junction protein Occludin and ZO-1 and investigate the role of Occludin, ZO-1 in the development of chronic gastritis. Methods Twenty-five healthy people with normal gastric mucosa, 30 patients pathologically confirmed H. priori-positive with chron- ic superficial gastritis, 30 patients with atrophic gastritis were detected by rapid urease test. Expressions of Occludin and ZO-1 protein were examined by immunohistochemical staining method in normal gastric mucosa and chronic gastritis mu- cosa before and after tt. priori eradication. Results The expressions of Occludins and ZO-1 were up-regulated in normal gastric mucosa without H. priori infection compared with H. priori-infected gastric mucosa with chronic gastritis （P 〈 0.05）. In atrophic gastritis mucosa, expressions of Occludin and ZO-1 were significantly decreased compared with superficial gastritis mucosa （P 〈 0.05）. The expressions of Occludin and ZO-1 in superficial gastritis were obviously increased after H. priori eradication （ P 〈 0.05 ）, however, there was no significant difference in atrophic gastritis after H. priori eradication （P 〉 0. 05 ）. There was also no significant change in those patients whose H. priori were still positive after H. priori eradication （P 〉 0.05）. Conclusion The expressions of Occludin and ZO-1 in gastric mucosa are associated with the development of chronic gastritis caused by H. priori infection. Eradication of H. priori before atrophic can increase the expressions of Occludin and ZO-1 in gastric mucosa and inhibit the development of gastritis.

关 键 词：慢性胃炎 幽门螺杆菌 紧密连接蛋白 

分 类 号：R573.3[医药卫生—消化系统]