ACAT1基因干扰对人结肠癌细胞增殖及侵袭转移的影响  

Effect of silencing ACAT1 gene by RNA interference on proliferation,migration and invasion of human colon cancer cells

作　　者：陈心[1] 梁慧玲[1] 徐细明[1] 张一桥[1] 陈能[1] 林丹丹[1] 冯翎[1] 郑勇斌[2] 

机构地区：[1]武汉大学人民医院肿瘤科,430060 [2]武汉大学人民医院胃肠外科,430060

出　　处：《实用医学杂志》2017年第7期1074-1077,共4页The Journal of Practical Medicine

基　　金：中央高校基本科研业务费专项基金资助项目(编号:2042014kf0149);湖北省自然科学基金资助项目(编号:2012FKC143)

摘　　要：目的:利用小干扰RNA(si RNA)技术干扰酰基辅酶A-胆固醇酰基转移酶1(ACAT1)基因在人结肠癌HT29细胞中的表达,观察ACAT1基因表达下降对于人结肠癌HT29细胞增殖和侵袭迁移能力的影响,了解ACAT1在结肠癌发生发展中的作用。方法:通过脂质体Hiper Fect将ACAT1 si RNA转染至人结肠癌HT29细胞中干扰ACAT1基因的表达,利用Real time PCR检测转染前后ACAT1基因表达的变化,利用ACAT1基因干扰前后的HT29细胞,采用CFSE染色法检测HT29细胞增殖能力的变化;Transwell小室法检测HT29细胞侵袭和迁移能力的变化。结果:ACAT1 si RNA转染HT29细胞后ACAT1 m RNA的表达明显下降,ACAT1 si RNA转染后的HT29细胞与转染前比较,增殖及侵袭转移的能力均受到明显抑制,差异有统计学意义(P<0.05)。结论:ACAT1基因干扰使HT29细胞的增殖减慢,侵袭迁移能力降低,为结肠癌治疗提供新的靶点。Objective To evaluate the effect of silencing ACAT1 gene on colon cancer cells proliferation, migration, invasion and colon cancer development by using the small interference RNA （siRNA） in colon cancer cell line HT-29. Methods Acyl coenzyme A cholesterol acyltransferase 1 （ACATI） gene was silenced in HT-29 cell lines using Hiperfect transfection reagent. The expression level of ACAT1 was detected by real time PCR. CFSE and transwell assays were used to evaluate the effect of ACAT1 gene interfering on cells proliferation, migration and invasion. Result ACAT1 mRNA expression decreased obviously after siRNA interference. Compared with pre-transfection, proliferation, migration and invasion of colon cancer cells have been significantly inhibited （P 〈 0.05）. Conclusion ACAT1 gene interference reduced proliferation, migration and of invasion of HT29 cells, which provide a new potential target for colon cancer treatment.

关 键 词：结肠肿瘤 酰基辅酶A-胆固醇酰基转移酶1 小干扰RNA 

分 类 号：R735.35[医药卫生—肿瘤]