非转移性结直肠癌组织中MMR的表达状态及意义  

Expression of MMR in non-metastatic colorectal carcinoma

作　　者：岳欣[1] 胡均[1] 王家仓[1] YUE Xin HU Jun WANG Jiacang(Tianjin Medical University Cancer Hospital, Tianjin 300060, Chin)

机构地区：[1]天津医科大学肿瘤医院,天津300060

出　　处：《山东医药》2017年第9期9-11,共3页Shandong Medical Journal

基　　金：国家自然科学基金项目(81101870);天津市卫生局科技基金项目(2014KZ080)

摘　　要：目的观察错配修复基因(MMR)在非转移性结直肠组织中的表达状态并探讨其意义。方法选取141例接受手术治疗的非转移性结直肠癌患者,均未接受术前放疗或化疗。术后病理标本进行常规病理检测及免疫组化法检测4种MMR蛋白(MLH1、MSH2、MSH6、PMS2)表达。四种蛋白中一种及以上不表达,即判定为MMR表达缺失(d MMR),对比MMR正常和d MMR结直肠癌患者的临床病理特征。结果非转移性结直肠癌患者的MLH1、MSH2、MSH6、PMS2表达缺失率分别为15.6%(22/141)、2.8%(4/141)、7.1%(10/141)、15.6%(22/141),d MMR率为25.5%(36/141)。MMR表达与非转移性结直肠癌患者年龄、部位、肿瘤大小及病理类型等有关(P均<0.05),d MMR更多发生于60岁以下、近端部位、黏液类型癌及较大的肿瘤(P均<0.05)。结论非转移性结直肠癌患者癌组织中MMR表达存在缺失,MMR状态的评估有助于判断患者预后和指导治疗。Objective To investigate the expression and significance of mismatch repair gene（ MMR） in non-metastatic colorectal tissues. Methods Totally 141 patients with non-metastatic colorectal cancer who underwent surgery were selected,and none of them received preoperative radiotherapy or chemotherapy. The expression of four MMR proteins（ MLH1,MSH2,MSH6,PMS2） in postoperative pathological specimens were detected by routine pathological examination and immunohistochemistry. One or more of the four proteins were not expressed,namely,the MMR expression was deleted（ d MMR）,and the clinicopathological features of the patients with normal and d MMR colorectal cancer were compared. Results The deletion rates of MLH1,MSH2,MSH6 and PMS2 were 15. 6%（ 22/141）,2. 8%（ 4/141）,7. 1%（ 10/141）,15. 6%（ 22/141）,and the d MMR rate was 25. 5%（ 36/141） in patients with non-metastatic colorectal cancer.MMR expression was correlated with non-metastatic colorectal cancer patient ＇s age,location,tumor size and pathological type（ all P〈0. 05）,and d MMR occurred more frequently in patients younger than 60 years,proximal,mucinous and large tumors（ all P〈0. 05）. Conclusion MMR expression is absent in non-metastatic colorectal cancer,and MMR status is helpful in predicting prognosis and guiding therapy.

关 键 词：结直肠癌 错配修复基因 微卫星不稳定性 染色体不稳定性 

分 类 号：R735.3[医药卫生—肿瘤]