苦杏仁中β-葡萄糖苷酶活性测定条件的研究  

Determination of β-glucosidase activity in Semen Armeniaceae Amarum

作　　者：赵丽莹[1] 拱健婷 米文娟[1] 李阳[1] 徐东[1] 赵婷[1] 闫永红[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中南药学》2017年第2期166-169,共4页Central South Pharmacy

基　　金：国家自然科学基金资助项目(No.81573542;No.81403054);北京中医药大学在读研究生资助项目(No.2016-JYB-XS128)

摘　　要：目的建立苦杏仁中β-葡萄糖苷酶活性测定的最优条件,为进一步阐明苦杏仁炮制原理及储藏养护条件研究提供依据。方法采用比色法,以对硝基苯-β-D-葡萄糖苷为底物,考察苦杏仁中β-葡萄糖苷酶活性测定的最佳条件。结果苦杏仁中β-葡萄糖苷酶活性测定的最佳条件为p H=5.0柠檬酸-磷酸氢二钠缓冲液,反应温度50℃,底物浓度50 mmol·L^(-1),反应时间30 min,于400 nm波长条件下检测β-葡萄糖苷酶活性。结论确定了测定苦杏仁中β-葡萄糖苷酶活性的最佳条件,为进一步研究苦杏仁"杀酶保苷"炮制原理、储藏养护条件及储藏期奠定基础。Objective To establish the optimal conditions for activity assay of β-glucosidase in Semen Armenia- ceae Amarum （Prunus armeniaca L. var. ansu Maxim.）. Methods The determination conditions of β-glucosidase activity in Semen Armeniaceae Amarum were colorimetric method with p-nitrophany-β-D-gluopyranoside （pNPG） as the substrate. Results The best determination conditions were as follows： pH value was 5.0, the substrate concentration was 50 mmol ·L-1, and then analyzing the enzyme activity with 400 nm wavelength at 50 ℃ after 30 min reaction. Conclusion The optimum conditions for activity study of β-glucosidase in Semen Armeniaceae Amarum are determined, which helps further study the processing principle and storage evaluation of Semen Armeniaceae Amarum.

关 键 词：苦杏仁 Β-葡萄糖苷酶 酶活性 比色法 

分 类 号：R284.1[医药卫生—中药学] TS261.7[医药卫生—中医学]