金莲花化学成分的分离与结构鉴定  

Isolation and identification of chemical constituents from Trollius chinensis Bunge

作　　者：师帅[1] 张建培[1] 刘婷[1] 马青苗 刘琛楠 张国刚[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2017年第4期297-301,316,共6页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省2016年度大学生创新创业训练计划项目(201610163026)

摘　　要：目的研究金莲花(Trollius chinensis Bunge)中的化学成分。方法采用大孔吸附树脂、正相硅胶、ODS、Sephadex LH-20等柱色谱法和制备型高效液相色谱法等分离技术对金莲花体积分数为60%的乙醇溶液提取物进行分离、纯化,并通过化合物的理化性质与波谱特征鉴定其结构。结果从体积分数为60%的乙醇溶液提取物中分离并鉴定了10个化合物,分别为cirsimarin(1)、icariside B6(2)、(+)-(R)-腈甲基-3-羟基氧吲哚[(+)-(R)-cyanomethyl-3-hydroxyoxindole,3]、黑麦草内酯(loliolide,4)、腺嘌呤(adenine,5)、3,4-二羟基苯甲醛(3,4-dihydroxybenzaldehyde,6)、金合欢素-7-O-β-D-葡萄糖苷(acacetin-7-O-β-D-glucoside,7)、2"-O-(3''',4'''-二甲氧基苯甲酰基)牡荆苷[2"-O-(3''',4'''-dimethoxybenzoyl)vitexin,8]、pinoresinol-β-D-glucopyranoside(9)、芹菜素-8-C-(2"-O-阿魏酰基)-β-D-葡萄糖苷[apigenin-8-C-(2"-O-feruloyl)-β-D-glucoside,10]。结论化合物1-6为首次从金莲花属植物中分离得到。Objective To study the chemical constituents from Trollius chinensis Bunge.Methods The compounds were isolated from 60%（φ） ethanol extract of Trollius chinensis Bunge by chromatographic methods such as macroporous resin,silica gel,ODS,Sephadex LH-20 column chromatography,HPLC and so on.The compounds were identified by the physicochemical property and spectral features.Results Ten compounds were isolated from 60%（φ） ethanol extract and their structures were identified as cirsimarin（1）,icariside B6（2）,（＋）-（R）-cyanomethyl-3-hydroxyoxindole（3）,loliolide（4）,adenine（5）,3,4-dihydroxybenzaldehyde（6）,acacetin-7-O-β-D-glucoside（7）,2＂-O-（3＇＇＇,4＇＇＇-dimethoxybenzoyl） vitexin（8）,pinoresinol-β-D-glucopyranoside（9）,apigenin-8-C-（2＂-O-feruloyl）-β-D-glucoside（10）,respectively.Conclusions Compounds 1-6 are obtained from the genus of Trollius for the first time.

关 键 词：金莲花 化学成分 结构鉴定 

分 类 号：R914[医药卫生—药物化学]