猪Leptin基因多态性及其与产仔性状的相关性分析  

The Association Analysis Between the SNPs of Leptin Gene and Litter Size Trait in Pigs

作　　者：朱爱文[1] 周春宝[1] 倪黎纲[1] 韩大勇[1] 

机构地区：[1]江苏农牧科技职业学院,泰州225300

出　　处：《中国畜牧兽医》2017年第4期1122-1127,共6页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省农业三新工程项目(SXGC[2014]271);江苏农牧科技职业学院青年科研基金(QN1214);江苏高校品牌专业建设工程资助项目(PPZY2015A083)

摘　　要：试验旨在探究Leptin基因对猪产仔性状的影响,为其新品种持续选育提供遗传学依据。参考GenBank发布的猪Leptin基因DNA序列(登录号:U66254、AF026976)设计3对引物,采用PCR-SSCP技术分析Leptin基因在苏姜猪和姜曲海猪中的单核苷酸多态性(SNP),并用统计学方法分析基因座多态性与产仔性状的相关性。结果发现,Leptin基因在苏姜猪、姜曲海猪群体中均具有多态性,检测到3种基因型:AA、AB和BB;测序结果发现,编码区第3 469位碱基处发生单碱基突变(T3469C),为同义突变。χ2适合性检验显示,2个猪种Leptin基因型分布处于Hardy-Weinberg平衡(P>0.05)。多态信息含量(PIC)分析显示,苏姜猪为中度多态,姜曲海猪为低度多态。Leptin基因型与产仔性状关联分析显示,不同基因型间产仔性状差异不显著(P>0.05),但T3469C位点AB基因型平均产仔数较AA和BB基因型高,初步推断Leptin基因T3469C位点可能是影响猪产仔性状潜在的遗传标记,建议做进一步的研究。The research was aimed to analyze the association of Leptin gene and breed traits on pigs. Three pairs of primers were designed which based on DNA sequence of pig Leptin gene （GenBank accession No. U66254, AF026976）, the single nucleotide polymorphisms （SNPs） of Leptin gene were detected by PCR-SSCP in Sujiang and Jiangquhai pigs. The results showed that three genotypes of AA,AB and BB were identified, and the mutation （T3469C） in CDS of Leptin gene was found, but amino acid change weren＇t happened. The Chi-square test results showed that the Leptin genotype distribution of Sujiang and Jiangquhai pigs was in Hardy-Weinberg equilibrium （P〉0.05）. The polymorphism information content （PIC） were moderate polymorphism in Sujiang pigs,and low polymorphism in Jiangquhai pigs. The statistic analysis results showed that there were no significant association between the SNP of Leptin genotype and litter size trait （P〉0.05）. But the average litter size of T3469C site AB genotype was higher than AA and BB genotypes, it was concluded that the T3469C polymorphism of Leptin gene might be the genetic marker which potentially affected pig breed traits.

关 键 词：苏姜猪 姜曲海猪 LEPTIN基因 PCR-SSCP 产仔性状 

分 类 号：S814.8[农业科学—畜牧学]